Bioactive, Recombinant Human Nectin-3 / CD113 Protein Dimer, His-Avi Tag
| Product Code | CSP-25196-03 |
| Expression Host | HEK293T |
| Verified Applications | ELISA for Nectin-3-specific antibody binding assays and SPR for Nectin-1 ligand protein binding assays. |
| Suggested Applications | SPR & BLI for Nectin-3-specific antibody binding assays and BLI and ELISA for Nectin-1 protein binding assays. Animal immunization, RUO. |
| Purity | Greater than 90% dimer form as determined by SDS-PAGE under non-reducing condition |
| Amino Acid Range | 1: G58-T404 |
For Research Use Only (RUO)
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Antibody Binding
Immobilized human Nectin-3 protein dimer, His-Avi tag (Cat. No. CSP-25196-03) at 2 μg/mL (100 μL/well) can bind anti-human Nectin-3 monoclonal antibody with half maximal effective concentration (EC50) range of 82-327.8 ng/mL (QC tested).
SDS-PAGE
NR: Nectin-3 dimer under non-reduced condition
Label Free Assay
Immobilized human Nectin-1 protein dimer, His-Avi tag (Cat. No. CSP-25195-03) can bind human Nectin-3 protein dimer, His-Avi tag (Cat. No. CSP-25196-03) with a KD of 3-11.8 nM as determined by LSPR (Nicoya Alto).
Specifications
Formulation: 0.22μm filtered PBS, pH 7.4
Shipping: Frozen Dry Ice
Storage: -80°C
Human Nectin-3 is a type 1 integral membrane glycoprotein that can form a homo-cis dimer on the cell membrane. The recombinant Nectin-3 dimer protein (CSP-25196-03) is a cis-homodimer (cis-dimer) and contains a Nectin-3 extracellular domain (UniProt# Q9NQS3, amino acids Gly58-Thr404) fused with a proprietary dimer motif followed by a tandem His-Avi tag at the C-terminus. This dimeric protein is expressed in HEK293T cells. The recombinant human Nectin-3 dimer protein is bioactive and can bind to Nectin-1. It also binds Nectin-3-specific antibodies. This Nectin-3 dimer can be used as an antigen for in vitro assays and antibody screening, and as an immunogen for immunization to generate antibodies targeting more conformational epitopes.
Protein Name: NECTIN3
UniProt #: 1: Q9NQS3
Predicted Molecular Weight: 96 kDa
SDS PAGE Molecular Weight: The migration range of the heterodimer protein with glycosylation under non-reducing condition is ~190 kDa on SDS PAGE.
Protein Construct: Nectin-3 dimer protein contains a Nectin-3 extracellular domain (UniProt# Q9NQS3) fused with a proprietary cis-dimer motif followed by a tandem His-Avi tag at the C-terminus.
Background
Human Nectin-3 is a type 1 integral membrane glycoprotein that can form a homo-cis dimer on the cell membrane. Nectin-3 is also known as poliovirus receptor-related 3 (PVRL3) and cluster of differentiation 113 (CD113). Nectin-3 contains an extracellular domain with three immunoglobulin-like (Ig-like) domains. Nectin-3 interacts with Nectin-1. Nectin-1 and Nectin-3 are expressed in neuronal tissue and unlike many other cellular adhesion molecules, they do not distribute evenly. Nectin-3 is primarily found on the dendritic side and Nectin-1 is primarily found on the axonal side. The interaction of Nectin-1 with Nectin-3 has been found to be essential for proper morphogenesis of the eye. Additionally, Nectin-3 is highly expressed in epithelial cancer cells of 80% of patients with human lung adenocarcinoma. Therefore, a recombinant protein mimicking the Nectin-3 dimer conformation can be crucial for cancer therapeutic discovery.
Alternate Names: poliovirus receptor-related 3, PVRL3, cluster of differentiation 113, CD113, nectin3, CDW113, PPR3, PRR3, PVRR3, nectin cell adhesion molecule 3