Immobilized human CX3CR1 VLP (Cat. No. CMP-25307) at 5 μg/mL (100 μL/well) can bind monoclonal anti-human CX3CR1 antibody (Cat. No. CABh-25316) with half maximal effective concentration (EC50) range of 1-9 μg/mL (QC tested).
Immobilized human CX3CL1 protein at 5 μg/mL (100 μL/well) can bind human CX3CR1 VLP(Cat. No. CMP-25307) with half maximal effective concentration (EC50) range of 2.9-11.4 μg/mL detected with monoclonal anti-human CX3CR1 antibody (Cat. No. CABh-25316) (QC tested).
The mean peak diameter (nm) of the CMP is 166 with a range of 156.-176 with more than 95% intensity as determined by dynamic light scattering (DLS).

Human CX3CR1 Full Length Protein, Displayed on Virus-Like Particles (VLPs)

Product Category: GPCR

Product Code: CMP-25307
Expression Host: HEK293T
Verified Applications: ELISA for CX3CR1-specific antibody binding assays.
Suggested Applications: Animal immunization, RUO.
Amino Acid Range: Met1 – Leu355

For Research Use Only (RUO)

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Formulation: 0.45μm filtered PBS, pH 7.4
Shipping: Frozen Dry Ice
Storage: On receipt, store at -70 C or lower for long term storage. This product is stable at: -70 C for 6 months from date of receipt. Avoid repeated freeze-thaw cycles

Datasheet

MSDS

Human CX3CR1 (CX3C motif chemokine receptor 1) is a is a transmembrane protein and a member of the G protein-coupled receptor 1 (GPCR1) family and the only known member of the CX3C chemokine receptor subfamily. The human CX3CR1 CMP (CMP-25307) is expressed on HEK293T cells and contains full length CX3CR1 transmembrane protein (UniProt# P49238, amino acids Met1 – Leu355) displayed on Virus-Like Particles (VLPs) and mimics the native protein conformation on the cell surface. The human CX3CR1 integral membrane protein is bioactive and can bind to anti-human CX3CR1 antibodies. This Virus-Like Particle (VLP) can be used as an antigen for in vitro assays and antibody screening, and as an immunogen for immunization to generate antibodies targeting conformational epitopes.
Product Name: CX3CR1, VLP
UniProt #: P49238
Predicted Molecular Weight: N/A
Protein Construct: CX3CR1 full length protein, displayed on Virus-Like Particlesmimics the native protein conformation on the cell surface.

Background and Alternate Names

Human CX3CR1 (CX3C motif chemokine receptor 1) is a seven-transmembrane protein and a member of the G protein-coupled receptor 1 (GPCR1) family. It is the only known member of the CX3C chemokine receptor subfamily. CX3CR1 is also known as fractalkine receptor or G-protein coupled receptor 13 (GPR13). CX3CR1 integral membrane protein containing three intracellular loops and an internal C-terminus with a conserved DRYLAIV motif for binding the heterotrimeric G protein, seven transmembrane domains, and three extracellular loops and extracellular N-terminus. CX3CR1 is expressed on numerous cell types, including various cells from hematopoietic lineage such as T lymphocytes, natural killer cells, and macrophages. Interaction of CX3CR1 with its ligand CX3CL1 mediates recruitment of immune cells by migration through chemotaxis and diapedesis as well as mediating migration, adhesion and retention of leukocytes. CX3CR1 increases accumulation of immune cells in affected areas of the body, which can result in aggravation of several diseases such as Rheumatoid arthritis, Renal diseases, Chronic liver disease, and Crohn’s disease. CX3CR1 is also a coreceptor for HIV-1, and some CX3CR1 gene variants lead to increased susceptibility to HIV-1 infection and quicker progression to AIDS.

Alternate Names: fractalkine receptor, G-protein coupled receptor 13, GPR13, CCRL1, CMKBRL1, CMKDR1, GPR13, GPRV28, V28, C-X3-C motif chemokine receptor 1