Immobilized human LGR4 at 2 μg/mL (100 μL/well) can bind Mouse Anti-Human LGR4 Monoclonal Antibody (Cat. No. CABh-25292) with half maximal effective concentration (EC50) range of 19.6-78.2 ng/mL (QC tested).
Detection of LGR4 in HEK293 human cell line transiently transfected with human LGR4 by Flow Cytometry. HEK293 cell line transfected with human LGR4 was stained with isotype control (black curve) or Mouse Anti-Human LGR4Monoclonal Antibody (Cat. No. CABh-25292) (green curve) by APC-conjugated Anti-Mouse IgG Secondary Antibody.
Immobilized human LGR4 protein can bind Mouse Anti-Human LGR4 Monoclonal Antibody (Cat. No. CABh-25292) with a KD of 0.1-0.4 nM as determined by LSPR (Nicoya Alto).

Anti-Human LGR4 Monoclonal Antibody

Product Category: Antibody

Product Code: CABh-25292
Isotype: IgG2a
Conjugate: Unconjugated
Specificity: Human Leucine-rich repeat-containing G-protein coupled receptor 4/LGR4 shows low affinity binding to LGR4 transfected cells by flow cytometry.
Host Species: Mouse
Immunogen: LGR4 N-terminus, the exact sequence of the immunogen is proprietary.; UniProt # Q9BXB1
Purification: Affinity Enrichment
Expression Host: HEK293T
Verified Applications: ELISA, SPR, Flow cytometry
Recommended Usage: ELISA: starting concentration 1 µg/mL. LSPR: Recommended maximum concentration, 100 nM.

For Research Use Only (RUO)

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Formulation: 0.22μm filtered PBS, pH 7.4
Shipping: Frozen Dry Ice
Storage: -80’C

Datasheet

MSDS

Structure: 7-transmembrane protein, G protein-coupled receptor (GPCR)
Function: Activator of the Wnt signaling pathway required for the development of various organs
Ligand/Receptor: R-spondins
Cell Type: Variable expression in most tissues; over-expressed on cancer cells, for example, lung cancer adenocarcinomas
Molecular Family: G protein-coupled receptor
Gene ID: Q9BXB1
Product Name: LGR4, Monoclonal Antibody
UniProt #: Q9BXB1
Predicted Molecular Weight: N/A
Protein Construct: N/A

Background and Alternate Names

Human Leucine-rich repeat-containing G-protein coupled receptor 4 (LGR4), also called BNMD17, GPR48, and DPSL, is a 7-transmembrane G protein-coupled receptor (GPCR). It is a Class B GPCR. Upon binding R-spondins, LGR4 triggers the canonical Wnt signaling pathway to increase expression of target genes. Unlike most classical G-protein coupled receptors, LGR4 does not activate heterotrimeric G-proteins to transduce the signal. LGR4 is a potential diagnostic biomarker and therapeutic target due to its promotion of tumorigenesis in some cancers. Human LGR4 protein has ~97% and ~93% sequence homology with macaque and mouse LGR4, respectively.

Alternate Names: BNMD17, GPR48, DPSL