Immobilized human GCGR fragment at 2 μg/mL (100 μL/well) can bind mouse Anti-Human GCGR Monoclonal Antibody (Cat. No. CABh-25374) with half maximal effective concentration (EC50) range of 16.9-67.6 ng/mL (QC tested).

Anti-Human GCGR Monoclonal Antibody

Product Category: All Products

Product Code: CABh-25374
Isotype: IgG1
Specificity: Human
Host Species: Mouse
Immunogen: GCGR N-terminus, the exact sequence of the immunogen is proprietary.; UniProt # P47871
Purification: Affinity Enrichment
Expression Host: HEK293T
Verified Applications: ELISA
Recommended Usage: ELISA: starting concentration 1 µg/mL.

For Research Use Only (RUO)

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Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4 with 10% trehalose as a protectant
Shipping: Frozen Ice Packs
Storage: On receipt, store at -20 C or lower for long term storage. This product is stable at: -20 to -70 C for 12 months from date of receipt in lyophilized state. -70 C for 3 months after reconstitution under sterile conditions. Avoid repeated freeze-thaw cycles
Reconstitution Information:

For best performance, we strongly recommend you reconsitute the lyophilized product with deionized water to a stock solution of 500 µg/mL.
Solubilize for 20 minutes at room temperature with occasional gentle mixing. Avoid shaking or vortexing.

For 25µg product size:
Reconstitute with 50 µL sterile deionized water.

For 100µg product size:
Reconstitute with 200 µL sterile deionized water.

Datasheet

MSDS

Structure: 7-transmembrane protein, G protein-coupled receptor (GPCR)
Function: regulation of blood glucose levels and glucose homeostasis
Ligand/Receptor: Glucagon
Cell Type: hepatocytes, renal nephron cells, adipocytes, and pancreatic islet α/β cells
Molecular Family: G protein-coupled receptor
Gene ID: P47871
Product Name: GCGR, Monoclonal Antibody
UniProt #: P47871
Predicted Molecular Weight: N/A
Protein Construct: N/A

Background and Alternate Names

Human glucagon receptor (GCGR) is a 7-transmembrane G protein-coupled receptor (GPCR). GCGR is a class B GPCR. It plays a key role in glucose homeostasis and the pathophysiology of type 2 diabetes. GCGR is expressed in many tissues but most highly expressed in the liver and kidney including hepatocytes, renal nephron cells, adipocytes, and pancreatic islet α/β cells. Upon ligand binding a conformation change triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase to regulate blood glucose levels and glucose homeostasis. Mutations in GCGR are associated with higher risk of hypertension and obesity. Human GCGR protein has ~80% sequence homology with mouse GCGR.

Alternate Names: GGR, GL-R, MVAH