Immobilized human GPR75 fragment at 2 μg/mL (100 μL/well) can bind mouse Anti-Human GPR75 Monoclonal Antibody (Cat. No. CABh-25401) with half maximal effective concentration (EC50) range of 1.5-6 ng/mL (QC tested).
Detection of GPR75 in HEK293 human cell line transiently transfected with human GPR75 by Flow Cytometry. HEK293 cell line transfected with human GPR75 was stained with isotype control (black curve) or mouse Anti-Human GPR75 Monoclonal Antibody (Cat. No. CABh-25401) (green curve) by APC-conjugated Anti-Mouse IgG Secondary Antibody.

Anti-Human GPR75 Monoclonal Antibody

Product Category: GPCR

Product Code: CABh-25401
Isotype: IgG2b
Specificity: Detects human probable G-protein coupled receptor 75/GPR75 transfectants but not irrelevant transfectants in flow cytometry.
Host Species: Mouse
Immunogen: GPR75 N-terminus extracellular domain, the exact sequence of the immunogen is proprietary.; UniProt # O95800
Purification: Affinity Enrichment
Expression Host: HEK293T
Verified Applications: ELISA, Flow cytometry
Recommended Usage: ELISA: starting concentration 1 µg/mL Flow cytometry: 0.8 µg/1E6 cells

For Research Use Only (RUO)

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Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4 with 10% trehalose as protectant
Shipping: Frozen Ice Packs
Storage: -20’C

Datasheet

MSDS

Structure: 7-transmembrane protein, G protein-coupled receptor (GPCR)
Function: Cellular signaling
Ligand/Receptor: Unknown
Cell Type: Various tissues and cell types, including neurons and Vascular endothelial & smooth muscle cells
Molecular Family: G protein-coupled receptor
Gene ID: O95800
Product Name: GPR75, Monoclonal Antibody
UniProt #: O95800

Background and Alternate Names

Human probable G-protein coupled receptor 75 ( GPR75), is a 7-transmembrane G protein-coupled receptor (GPCR). GPR75 is a Class A GPCR and plays critical roles in cellular signaling. GPR75 is expressed in several tissues, including the brain, where it is thought to be involved in neural signaling and metabolic regulation. GPR75 has gained increasing attention due to its proposed role in regulating energy balance and metabolism, reducing body mass index and protecting against obesity. GPR75 represents an important area of ongoing research, with promising implications for understanding and treating metabolic diseases. Human GPR75protein has 85-90% sequence homology with mouse GPR75.

Alternate Names: GPRchr2, WI31133, G protein-coupled receptor 75 IgG2b, clone # 2D4-H9