Immobilized human ACKR3 fragment at 2 μg/mL (100 μL/well) can bind Mouse Anti-Human ACKR3 Monoclonal Antibody (Cat. No. CABh-25380) with half maximal effective concentration (EC50) range of 5.1-20.4 ng/mL (QC tested).
Detection of ACKR3 expressed on HEK293 cell surface transiently transfected with full-length human ACKR3 by Flow Cytometry. HEK293 cell line transfected with human ACKR3 was stained with isotype control (black curve) or mouse Anti-Human ACKR3 Monoclonal Antibody (Cat. No. CABh-25380) (green curve) by APC-conjugated Anti-Mouse IgG Secondary Antibody.

Anti-Human ACKR3 Monoclonal Antibody

Product Code: CABh-25380
Isotype: IgG2a
Specificity: Detects human atypical chemokine receptor 3 ACKR3/CXCR7 transfectants but not irrelevant transfectants in flow cytometry.
Host Species: Mouse
Immunogen: ACKR3/CXCR7 N-terminus, the exact sequence of the immunogen is proprietary.; UniProt # P25106
Purification: Affinity Enrichment
Expression Host: HEK293T
Verified Applications: ELISA, Flow cytometry
Recommended Usage: ELISA: starting concentration 1 µg/mL Flow cytometry: 0.8 µg/1E6 cells

For Research Use Only (RUO)

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Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4 with 10% trehalose as protectant
Shipping: Frozen Ice Packs
Storage: -20’C
Reconstitution Information:

For best performance, we strongly recommend you reconsitute the lyophilized product with deionized water to a stock solution of 500 µg/mL.
Solubilize for 20 minutes at room temperature with occasional gentle mixing. Avoid shaking or vortexing.

For 25µg product size:
Reconstitute with 50 µL sterile deionized water.

For 100µg product size:
Reconstitute with 200 µL sterile deionized water.

Datasheet

MSDS

Structure: 7-transmembrane protein, G protein-coupled receptor (GPCR)
Function: ligand internalization and activation of MAPK signaling pathway
Ligand/Receptor: CXCL12/SDF-1, CXCL11, macrophage inhibitory factor (MIF), adrenomedullin (ADM), opioid peptides, viral chemokine vCCL2
Cell Type: monocytes, basophils, B-cells, umbilical vein endothelial cells (HUVEC) and B-lymphoblastoid cells with lower expression detected in CD4+ T-lymphocytes and natural killer cells
Molecular Family: G protein-coupled receptor, chemokine receptor
Gene ID: P25106
Product Name: ACKR3, Monoclonal Antibody
Human atypical chemokine receptor 3 (ACKR3) is also known as C-X-C chemokine receptor type 7 (CXCR-7) and G-protein coupled receptor 159 (GPR159). It is a 7-transmembrane G protein-coupled receptor (GPCR); a Class A GPCR. ACKR3/CXCR7 triggers signaling pathways solely mediated by β- arrestin. ACKR3/CXCR7 is known as an “interceptor” (internalizing receptor) or chemokine-scavenging receptor or chemokine decoy receptor resulting in chemokine sequestration. Upon chemokine binding to ACKR3/CXCR7 beta-arrestin recruitment is induced, leading to ligand internalization and activation of MAPK signaling pathway. ACKR3/CXCR7 is a therapeutic target due to being a coreceptor for human immunodeficiency viruses (HIV). Human ACKR3 protein has >90% sequence homology with mouse ACKR3.

Alternate Names: CMKOR1, CXC-R7, CXCR-7, CXCR7, GPR159, RDC-1, RDC1