MW: Molecular Weight marker reduced condition NR: B7H3-4lg dimer under non-reduced condition
Immobilized human B7H3-4lg-His dimer protein (CSP-260984-01) at 2 μg/mL (100 μL/well) can bind anti-human B7H3 monoclonal antibody with half maximal effective concentration (EC50) range of 24.2-96.8 ng/mL (QC tested).

Human 4Ig-B7H3 Protein Dimer, His Tag

Product Category: Tumor Associated Antigen

Product Code: CSP-260984-01
Specificity: Human
Expression Host: HEK293T
Verified Applications: ELISA for 4Ig-B7H3-specific antibody binding assay.
Suggested Applications: Animal immunization, RUO.
Purity: Greater than 90% dimer form as determined by SDS-PAGE under non-reducing condition
Amino Acid Range: L29 – A466

For Research Use Only (RUO)

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Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4 with 10% trehalose as protectant
Shipping: Frozen Ice Packs
Storage: -20’C

Datasheet

MSDS

Human B7 Homolog 3 (B7H3) isoform 4Ig-B7H3 (4Ig-B7-H3, 4IgB7H3) is a Type I membrane protein characterized by an extracellular domain containing two pairs of IgV-like and IgC-like immunoglobulin domains rather than the single pair like isoform 2Ig-B7H3 (2Ig-B7-H3, 2IgB7H3). B7H3 can form a dimer that is important for its function. The recombinant 4Ig-B7H3 dimer protein (CSP-260984-01) is a cis-homodimer (cis-dimer) and contains a 4Ig-B7H3 extracellular domain with two pairs of IgV-like and IgC-like immunoglobulin domains (UniProt# Q5ZPR3-1, amino acids Leu29-Ala466) fused with a proprietary dimer motif followed by a His tag at the C-terminus. This dimeric protein is expressed in HEK293 cells. The recombinant human 4Ig-B7H3 dimer protein is bioactive and can bind 4Ig-B7H3-specific antibodies. This 4Ig-B7H3 dimer can be used as an antigen for in vitro assays and antibody screening, and as an immunogen for immunization to generate antibodies targeting more conformational epitopes.
Product Name: B7H3 isoform 4lg, Protein Dimer
Predicted Molecular Weight: 114 kDa
SDS PAGE Molecular Weight: The migration range of the dimer protein with glycosylation under non-reducing condition is 190 kDa on SDS PAGE.
Protein Construct: 4Ig-B7H3 dimer protein contains a 4Ig-B7H3 extracellular domain with two pairs of IgV-like and IgC-like immunoglobulin domainsfused with a proprietary dimer motif followed by a His tag at the C-terminus.
Human B7 Homolog 3 (B7H3, B7-H3) is a member of the B7 family of immune checkpoint proteins involved in regulating immune responses. Human B7H3 has 2 isoforms: 2Ig-B7H3 (2Ig-B7-H3, 2IgB7H3) and 4Ig-B7H3 (4Ig-B7-H3, 4IgB7H3). B7H3 is a Type I membrane protein characterized by an extracellular domain, a transmembrane domain and a cytoplasmic domain. The 4Ig-B7H3 isoform contains two pairs of IgV-like and IgC-like immunoglobulin domains while the 2Ig-B7H3 isoform has a single pair of IgV-like and IgC-like immunoglobulin domains in the extracellular region. B7H3 is also known as B7-H3, Cluster of Differentiation 276 (CD276), and B7RP-2. B7H3 has limited expression on normal tissues but is highly expressed in many cancer cells including lung, kidney, ovarian, colorectal, liver, and breast cancers, which can contribute to immune evasion by inhibiting T cell activation. B7H3 can form a dimer that is critical for its function and can more effectively interact with its receptor(s) on T cells and other immune cells. B7H3 is overexpressed in up to 60% of all cancers and due to its selective expression on solid tumors B7H3 has become a critical target for cancer therapies.

Alternate Names: Cluster of Differentiation 276 (CD276), 4IgB7-H3, 4Ig-B7H3, B7-H3, B7H3, B7RP-2