MW: Molecular Weight marker reduced condition
NR: Siglec 6 dimer under non-reduced condition
Immobilized human Siglec 6 protein dimer,His-Avi Tag (CSP-26203-03) at 2 μg/mL (100 μL/well) can bind anti-human Siglec 6 monoclonal antibody with half maximal effective concentration (EC50) range of 12.1-48.4 ng/mL (QC tested).

Human Siglec 6 Protein Dimer, His-Avi Tag

Product Category: Siglec receptors

Product Code: CSP-26203-03
Specificity: Human
Expression Host: HEK293T
Verified Applications: ELISA for Siglec 6-specific antibody binding assays.
Suggested Applications: SPR & BLI for Siglec 6-specific antibody binding assays. Animal immunization, RUO.
Purity: Greater than 90% dimer form as determined by SDS-PAGE under non-reducing condition
Amino Acid Range: Q27 – V347

For Research Use Only (RUO)

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Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4 with 10% trehalose as protectant
Shipping: Frozen Ice Packs
Storage: -20’C
Reconstitution Information:

For best performance, we strongly recommend you reconsitute the lyophilized product with deionized water to a stock solution of 500 µg/mL.
Solubilize for 20 minutes at room temperature with occasional gentle mixing. Avoid shaking or vortexing.

For 25µg product size:
Reconstitute with 50 µL sterile deionized water.

For 100µg product size:
Reconstitute with 200 µL sterile deionized water.

Datasheet

MSDS

Human sialic acid-binding Ig-like lectin 6 (Siglec-6) is a Type I transmembrane protein and a member of the Siglec family and immunoglobulin superfamily. The recombinant Siglec-6 protein dimer (CSP-26203-03) is a cis-homodimer (cis-dimer) and contains a Siglec-6 extracellular domain (UniProt# O43699, amino acids Gln27-Val347) fused with a proprietary cis-dimer motif followed by a tandem His-Avi tag at the C-terminus. This dimeric protein is expressed in HEK293T cells. The recombinant human Siglec-6 dimer protein can potently bind Siglec-6-specific antibodies. This Siglec-6 dimer can be used as an antigen for in vitro assays and antibody screening, and as an immunogen for immunization to generate antibodies targeting more conformational epitopes.
Product Name: Siglec 6, Protein Dimer
Predicted Molecular Weight: 87 kDa
SDS PAGE Molecular Weight: The migration range of the protein dimer with glycosylation under non-reducing condition is 190 kDa on SDS PAGE.
Protein Construct: Siglec-6 protein dimer contains a Siglec-6 extracellular domainfused with a proprietary cis-dimer motif followed by a tandem His-Avi tag at the C-terminus.
Human sialic acid-binding Ig-like lectin 6 (Siglec-6) is a Type I transmembrane protein, a member of the Siglec family, part of the immunoglobulin superfamily, and a multifunctional immune regulator. Siglec-6 is also known as Cluster of Differentiation 327 (CD327), CD33-like (CD33L), CD33L1, CD33L2, and CDW327. Siglec-6 contains an extracellular domain with an N-terminal V-type immunoglobulin domain (Ig domain) and two C2-type Ig domains followed by a transmembrane domain and cytoplasmic signaling domain consisting of the immunoreceptor tyrosine-based inhibitory motif (ITIM). Siglec-6 is expressed on trophoblasts and highly expressed on mast cells from a variety of tissues. It binds sialylated glycans on cell surfaces or secreted proteins, modulating cell-cell interactions and immune responses. The presence of Siglec-6 on various kinds of leukemias has made it an emerging target for cancer therapeutics.

Alternate Names: Cluster of Differentiation 327, CD327, CD33-like, CD33L, CD33L1, CD33L2, CDW327, SIGLEC6, Siglec 6