MW: Molecular Weight marker reduced condition
NR: MICB dimer under non-reduced condition
Immobilized human MICB-His dimer protein (CSP-26328-01) at 2 μg/mL (100 μL/well) can bind anti-human MICB monoclonal antibody with half maximal effective concentration (EC50) range of 3.3-13 ng/mL (QC tested).

Human MICB Protein Dimer, His Tag

Product Code: CSP-26328-01
Specificity: Human
Expression Host: HEK293T
Verified Applications: ELISA for MICB-specific antibody binding assays.
Suggested Applications: SPR & BLI for MICB-specific antibody binding assays and CAR-T detection. Animal immunization, RUO.
Purity: Greater than 90% dimer form as determined by SDS-PAGE under non-reducing condition
Amino Acid Range: A23 – D309

For Research Use Only (RUO)

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Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4 with 10% trehalose as protectant
Shipping: Frozen Ice Packs
Storage: -20’C
Reconstitution Information:

For best performance, we strongly recommend you reconsitute the lyophilized product with deionized water to a stock solution of 500 µg/mL.
Solubilize for 20 minutes at room temperature with occasional gentle mixing. Avoid shaking or vortexing.

For 25µg product size:
Reconstitute with 50 µL sterile deionized water.

For 100µg product size:
Reconstitute with 200 µL sterile deionized water.

Datasheet

MSDS

Human MHC class I polypeptide-related sequence B (MICB) is a MHC class I family protein. It is a type 1 transmembrane protein. The recombinant MICB dimer protein (CSP-26328-01) is a cis-homodimer (cis-dimer) and contains a MICB extracellular domain (UniProt# Q29980, amino acids Ala23 – Asp309) fused with a proprietary cis-dimer motif followed by a His tag at the C-terminus. This dimeric protein is expressed in HEK293T cells. The recombinant human MICB dimer protein is bioactive and can bind to MICB-specific antibodies. This MICB dimer can be used as an antigen for in vitro assays and antibody screening, and as an immunogen for immunization to generate antibodies targeting more conformational epitopes.
Product Name: MICB, Protein Dimer
Predicted Molecular Weight: 82 kDa
SDS PAGE Molecular Weight: Migration range of the dimer under non-reducing condition is 120-190 kDa on SDS PAGE
Protein Construct: MICB dimer protein contains a MICB extracellular domainfused with a proprietary cis-dimer motif followed by a His tag at the C-terminus.
Human MHC class I polypeptide-related sequence B (MICB) is a MHC class I family protein. MICB is also known as PERB11.2. It is a type 1 transmembrane protein. MICB contains an extracellular domain with an α1α2α3 domain followed by a transmembrane domain and cytoplasmic domain. MICB is a stress-induced ligand for the activating receptor NKG2D (KLRK1). Like MICA, MICB plays a key role in immune surveillance by activating NK cells and cytotoxic T cells in response to cellular stress, infection, or malignant transformation. MICB expression is highly restricted in healthy tissues but is strongly induced in stressed, infected, and malignant cells.. MICB is highly detected in many tumor tissues which has made MICB an emerging target for immunotherapy and cell therapy.

Alternate Names: PERB11.2