MW: Molecular Weight marker reduced condition
NR: CD123 dimer under non-reducing condition
Immobilized human CD123 dimer protein,His tag (Cat. No. CSP-26321-01) at 2 μg/mL (100 μL/well) can bind anti-human CD123 monoclonal antibody with half maximal effective concentration (EC50) range of 8.4-33.6 ng/mL (QC tested).

Human CD123 Protein Dimer, His Tag

Product Code: CSP-26321-01
Specificity: Human
Expression Host: HEK293T
Verified Applications: ELISA for CD123-specific antibody protein binding assays.
Suggested Applications: SPR & BLI for CD123-specific antibody binding assays and CAR-T detection. Animal immunization, RUO.
Purity: Greater than 90% dimer form as determined by SDS-PAGE under non-reducing condition
Amino Acid Range: T19 – R305

For Research Use Only (RUO)

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Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4 with 10% trehalose as protectant
Shipping: Frozen Ice Packs
Storage: -20’C
Reconstitution Information:

For best performance, we strongly recommend you reconsitute the lyophilized product with deionized water to a stock solution of 500 µg/mL.
Solubilize for 20 minutes at room temperature with occasional gentle mixing. Avoid shaking or vortexing.

For 25µg product size:
Reconstitute with 50 µL sterile deionized water.

For 100µg product size:
Reconstitute with 200 µL sterile deionized water.

Datasheet

MSDS

Human cluster of differentiation 123 (CD123) is a member of type I cytokine receptor family. The recombinant CD123 dimer protein (CSP-26321-01) is a cis-homodimer (cis-dimer) and contains a CD123 extracellular domain (UniProt# P26951, amino acids Thr19 – Arg305) fused with a proprietary cis-dimer motif followed by a His tag at the C-terminus. This dimeric protein is expressed in HEK293T cells. The recombinant human CD123 dimer protein is bioactive and can bind CD123-specific antibodies. This CD123 dimer can be used as an antigen for in vitro assays and antibody screening, and as an immunogen for immunization to generate antibodies targeting more conformational epitopes.
Product Name: CD123, Protein Dimer
Predicted Molecular Weight: 82 kDa
SDS PAGE Molecular Weight: The migration range of the dimer protein with glycosylation under non-reducing condition is 120-190 kDa on SDS PAGE.
Protein Construct: CD123 dimer protein contains a CD123 extracellular domainfused with a proprietary cis-dimer motif followed by a His tag at the C-terminus.
Human cluster of differentiation 123 (CD123) is in the Type I cytokine receptor family. CD123 is also known as interleukin-3 receptor alpha (IL3RA). CD123 is a type I membrane protein containing an extracellular domain, followed by a transmembrane, and cytoplasmic region. Its extracellular structure contains one immunoglobulin (Ig)-like domain and two fibronectin type III (FnIII) domains. Upon interleukin-3 ligand binding to the extracellular domain, CD123 rapidly heterodimerizes with cluster of differentiation 131 (interleukin-3 receptor beta). CD123 is expressed on pluripotent progenitor cells and promotes proliferation and differentiation of hematopoietic cell lines. CD123 is highly expressed in acute myeloid leukemia subtypes which has made CD123 an emerging strategy in cancer therapy.

Alternate Names: IL3RA, IL3R, IL3RAY, IL3RX, IL3RY, hIL-3Ra, interleukin 3 receptor subunit alpha