MW: Molecular Weight marker reduced condition NR: CD4 dimer under non-reduced condition
Immobilized human CD4 dimer protein, His Tag (Cat. No. CSP-24004) at 2 μg/mL (100 μL/well) can bind anti-human CD4 monoclonal antibody with half maximal effective concentration (EC50) range of 3.46 – 13.85 ng/mL (QC tested).
humna CD4 dimer ELISAImmobilized human CD4 dimer protein, His Tag (Cat. No. CSP-24004) at 2 μg/mL (100 μL/well) can bind HIV-1 envelope glycoprotein gp120 with half maximal effective concentration (EC50) range of 11.45 – 45.8 ng/mL (QC tested). The gp120 D368R mutation diminishes the binding to CD4 dimer protein as expected.
Immobilized human gp120 protein can bind human CD4 protein dimer, His tag (Cat. No. CSP-24004) with a KD of 2.4-9.6 nM as determined by LSPR (Nicoya Alto).

Bioactive, Human CD4 Protein Dimer, His Tag

Product Category: T-cell Receptor

Product Code: CSP-24004
Expression Host: HEK293T
Verified Applications: ELISA for antibody binding and ELISA and SPR gp120 binding.
Suggested Applications: BLI binding measurements, B-cell sorting, immunogen for animal immunization RUO.
Purity: Greater than 90% dimer form as determined by SDS-PAGE under non-reducing condition
Amino Acid Range: G25-P396

For Research Use Only (RUO)

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Formulation: 0.22μm filtered PBS, pH 7.4
Shipping: Frozen Dry Ice
Storage: -80’C

Datasheet

MSDS

Human CD4 protein is also known as IL16 receptor (IL16R). CD4 dimer (CSP-24004) contains CD4 extracellular 4-domains (amino acids Lys26-Phe396, UniProt# A0A4Y5UGE4) with a homodimer motif and a His tag at the C-terminus. This protein is expressed in HEK293 cells. The CD4 dimer protein is bioactive and can bind to HIV-1 envelope glycoproteins (Env) from various subtypes with on average >10 fold binding activity increases compared to CD4 monomer protein. The HIV-1 Env binding to CD4 dimer can be diminished by a D368R mutation. CD4 dimer also increases the binding to CD4-specific antibodies. This CD4 dimer can be used as an immunogen for animal immunization (RUO) and as an antigen for in vitro assays and antibody screening targeting more conformations epitopes.
Product Name: CD4
UniProt #: P01730-Partial
Predicted Molecular Weight: 112 kDa
SDS PAGE Molecular Weight: Migration range of the dimer under non-reducing condition is 100-150kDa on SDS PAGE
Protein Construct: CD4 dimer contains CD4 extracellular 4-domainswith a homodimer motif and a His tag at the C-terminus.

Background and Alternate Names

CD4 is type 1 integral membrane glycoprotein protein on T cell surface, also known as known as T-cell surface antigen T4/Leu-3. CD4 contains an extracellular domain, a transmembrane domain and a cytoplasmic domain. The extracellular domain has 4 immunoglobulin-like (Ig-like) domains: one Ig-like V-type domain and three Ig-like C2-type domains. The CD4 extracellular domain is responsible for MHC class-II antigen/T-cell receptor interaction and T cell activation. CD4 is also known as interleukin 16 receptor (IL16R). The IL16 cytokine binds CD4 to activate a downstream signalling cascade. CD4 is also the primary receptor for the human immunodeficiency virus (HIV) envelope glycoprotein gp120 to mediate HIV infection and entry into host T cells, as the underlying cause of acquired immune deficiency syndrome (AIDS).

Alternate Names: IL16R