MW: Molecular Weight marker reduced condition NR: Nectin-4 dimer under non-reducing condition

Immobilized human Nectin-4 dimer protein (Cat. No. CSP-24016) at 2 μg/mL (100 μL/well) can bind anti-human Nectin-4 monoclonal antibody with half maximal effective concentration (EC50) range of 1.1 - 4.3 ng/mL (QC tested).

Immobilized human Nectin-4 dimer protein (Cat. No. CSP-24016) at 5 μg/mL (100 μL/well) can bind TIGIT-His-Avi dimer protein (Cat. No. CSP-24028-03)

Bioactive, Human Nectin-4 cis dimer, His Tag

Tumor Associated Antigen

Product Code: CSP-24016

Expression Host: HEK293T

Verified Applications: ELISA, Western blot for Nectin-4-specific antibody and TIGIT receptor protein binding assays; animal immunization, RUO

Suggested Applications: BLI, SPR, B cell sorting

Purity: Greater than 90% dimer form as determined by SDS-PAGE under non-reducing condition

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Formulation: 0.22μm filtered PBS, pH 7.4

Shipping: Frozen Dry Ice

Storage: -80’C

Datasheet

MSDS

Human Nectin-4 (nectin cell adhesion molecule 4) is also known as a poliovirus receptor-related-4 (PVRL-4). Nectin-4 protein cis-dimer (Nectin-4 dimer, CSP-24016) contains Nectin-4 extracellular 3 Ig-like domains (amino acids Gly32-Ser349, UniProt# Q96NY8) with a homodimer motif and a His tag at the C-terminus. This protein is expressed in HEK293 cells. The Nectin-4 dimer protein is bioactive and can bind to its receptor TIGIT protein with significantly increased binding potency compared with the Nectin-4 monomer. It also binds to Nectin-4-specific antibodies. This Nectin-4 dimer can be used as an immunogen for animal immunization (RUO) and as an antigen for in vitro assays and antibody screening, targeting more conformational epitopes.

Protein Name: Nectin-4

UniProt #: Q96NY8

Predicted Molecular Weight: 92 kDa

SDS PAGE Molecular Weight: Migration range of the dimer under non-reducing condition is 90-130 kDa on SDS-PAGE.

Protein Construct: Nectin-4 protein cis-dimercontains Nectin-4 extracellular 3 Ig-like domainswith a homodimer motif and a His tag at the C-terminus.

Background

Nectin-4, also known as PVRL-4, is a type 1 integral membrane glycoprotein that can form a homo-cis dimer on the cell membrane. It consists of an extracellular region with three immunoglobulin-like (Ig-like) domains, organized as a variable-like (Ig-V) domain followed by two constant-like (Ig-C) domains (V-C-C), one transmembrane region, and a cytoplasmic domain. Nectin-4 promotes tumor growth and is overexpressed in numerous tumor types such as breast, lung, ovarian, urothelial, colorectal, and pancreatic cancer. Nectin-4 is a ligand of TIGIT (T-cell immunoreceptor with Ig and ITIM domains). Nectin-4 is an emerging target of cancer therapeutics. A recombinant protein mimicking the Nectin-4 native dimer conformation can be crucial for therapeutic antibody and vaccine discovery.

Bioactive, Human Nectin-4 cis dimer, His Tag

Tumor Associated Antigen

Datasheet

MSDS

Background

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