Immobilized human DRD2 peptide at 1 μg/mL (100 μL/well) can bind Mouse Anti-Human DRD2 Monoclonal Antibody (Cat. No. CABh-24075) with half maximal effective concentration (EC50) range of 4.5-18 ng/mL (QC tested).
Detection of DRD2 in HEK293 human cell line transiently transfected with human DRD2 by Flow Cytometry. HEK293 cell line transfected with human DRD2 was stained with isotype control (black curve) or Mouse Anti-Human DRD2 Monoclonal Antibody (Cat. No. CABh-24075) (green curve) by APC-conjugated Anti-Mouse IgG Secondary Antibody.
Immobilized human DRD2 peptide can bind Mouse Anti-Human DRD2 Monoclonal Antibody (Cat. No. CABh-24075) with a KD of 0.1-0.4 nM as determined by LSPR (Nicoya Alto).

Anti-DRD2 Monoclonal Antibody

Product Category: Antibody

Product Code: CABh-24075
Isotype: IgG2a
Conjugate: Unconjugated
Specificity: Detects human Dopamine Receptor D2/DRD2 transfectants but not irrelevant transfectants in flow cytometry. Does not detect mouse DRD2 transfectants in flow cytometry.
Host Species: Mouse
Immunogen: DRD2 N-terminus, the exact sequence of the immunogen is proprietary.; UniProt # P14416
Purification: Affinity Enrichment
Verified Applications: ELISA, Flow cytometry, LSPR
Purity: >99% purity determined by SDS-PAGE under non-reducing condition
Recommended Usage: ELISA: starting concentration 1 µg/mL. Flow cytometry: 0.8 µg/1E6 cells LSPR: Recommended maximum concentration, 100 nM

For Research Use Only (RUO)

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Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4 with 10% trehalose as a protectant
Shipping: Product is shipped at ambient temperature.
Storage: On receipt, store at -20 C or lower for long term storage. This product is stable at: -20 to -70 C for 12 months from date of receipt in lyophilized state. -70 C for 3 months after reconstitution under sterile conditions. Avoid repeated freeze-thaw cycles

Datasheet

MSDS

Structure: 7-transmembrane protein, G protein-coupled receptor (GPCR)
Function: Inhibit adenylyl cyclase activity
Ligand/Receptor: EPB41L1, PPP1R9B, NCS-1
Cell Type: Anterior pituitary gland
Molecular Family: G protein-coupled receptor
Gene ID: P14416
Product Name: DRD2, Monoclonal Antibody
UniProt #: P14416;P61168
Predicted Molecular Weight: N/A
Protein Construct: N/A

Background and Alternate Names

Human dopamine receptor D2 (DRD2) is a G protein-coupled receptor (GPCR) on cell surface that inhibits adenylyl cyclase activity. DRD2 contains seven transmembrane domains and is highly expressed in the anterior pituitary gland of the brain. DRD2 is the main receptor for most antipsychotic drugs and mutations in the gene encoding DRD2 have been associated with myoclonus dystonia and schizophrenia.

Alternate Names: D2DR, D2R