Bioactive, Recombinant Human CSF2RA Protein Dimer, His-Avi Tag
| Product Code | CSP-25172-03 |
| Expression Host | HEK293T |
| Verified Applications | ELISA for CSF2RA-specific antibody and ELISA and SPR for granulocyte-macrophage colony-stimulating factor (GM-CSF) ligand protein binding assays. |
| Suggested Applications | SPR & BLI for CSF2RA-specific antibody and BLI for GM-CSF protein binding assays. Animal immunization, RUO. |
| Purity | Greater than 90% dimer form as determined by SDS-PAGE under non-reducing condition |
| Amino Acid Range | E23-G320 |
For Research Use Only (RUO)
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Bioactivity – Antibody Binding
Immobilized human CSF2Rα protein dimer, His Tag (CSP-25172-03) at 2 μg/mL (100 μL/well) can bind anti-human CSF2Rα polyclonal antibody with half maximal effective concentration (EC50) range of 48.3-193.1 ng/mL (QC tested).
Bioactivity – Ligand Binding
Immobilized human GM-CSF at 2 μg/mL (100 μL/well) can bind anti-human CSF2Rα protein dimer, His Tag (CSP-25172-03) with half maximal effective concentration (EC50) range of 29.5-117.8 ng/mL (QC tested).
SDS-PAGE
NR: CSF2Rα under non-reduced condition
Specifications
Formulation: 0.22μm filtered PBS, pH 7.4
Shipping: Frozen Dry Ice
Storage: -80°C
Human colony stimulating factor 2 receptor subunit alpha (CSF2RA) is a Type I transmembrane protein and a glycoprotein. The recombinant CSF2RA protein dimer (CSP-25172-03) is a cis-homodimer (cis-dimer) and contains a CSF2RA extracellular domain (UniProt# P15509, amino acids Glu23-Gly320) fused with a proprietary cis-dimer motif followed by a tandem His-Avi tag at the C-terminus. This dimeric protein is expressed in HEK293T cells. The recombinant human CSF2RA dimer protein is bioactive and can bind to granulocyte-macrophage colony-stimulating factor (GM-CSF). It also binds CSF2RA-specific antibodies. This CSF2RA dimer can be used as an antigen for in vitro assays and antibody screening, and as an immunogen for immunization to generate antibodies targeting more conformational epitopes.
Protein Name: CSF2RA
UniProt #: P15509
Predicted Molecular Weight: 89 kDa
SDS PAGE Molecular Weight: The migration range of the heterodimer protein with glycosylation under non-reducing condition is 120~190 kDa on SDS PAGE.
Protein Construct: CSF2RA dimer protein contains a CSF2RA extracellular domain (UniProt# P15509) fused with a proprietary cis-dimer motif followed by a tandem His-Avi tag at the C-terminus.
Background
Human colony stimulating factor 2 receptor subunit alpha (CSF2RA) is a Type I transmembrane glycoprotein. CSF2RA is also known as granulocyte-macrophage colony-stimulating factor receptor alpha (GMCSFR-alpha), GMRα subunit, and cluster of differentiation 116 (CD116). The CSF2RA extracellular domain contains two fibronectin type III (FN III) domains with a highly conserved WSXWS motif that is present near the C-terminus of the second domain. CSF2RA is expressed on (cell types). CSF2RA is primarily located on neutrophils, eosinophils and monocytes/macrophages and is a receptor for granulocyte-macrophage colony-stimulating factor (GM-CSF). CSF2RA can form a heterodimer with CSF2RB and these heterodimers can form several multimers including a dodecamer. It has been found that CSF2RA specifically has tumor suppression characteristics that have made stimulating CSF2RA expression an emerging strategy in cancer therapy.
Alternate Names: granulocyte-macrophage colony-stimulating factor receptor alpha, GMCSFR-alpha, GMRα subunit, Cluster of Differentiation 116, CD116, CDw116, CSF2R, CSF2RAX, CSF2RAY, CSF2RX, CSF2RY, GM-CSF-R-alpha, GMCSFR, GMR, SMDP4, alphaGMR, GMR-alpha