MW: Molecular Weight marker reduced condition NR:
Immobilize human IFNγR1 dimer protein (Cat. No. CSP24015) at 2 μg/mL (100 μL/well) can bind anti-human IFNγR1 monoclonal antibody with half maximal effective concentration (EC50) range of 9.2-36.9 ng/mL (QC tested).
Immobilize human IFNγ protein at 2 μg/mL (100 μL/well) can bind IFNγR1 dimer protein (Cat. No. CSP-24015) with half maximal effective concentration (EC50) range of 0.95 - 3.8 ng/mL (QC tested).

Bioactive, Human IFNγ R1 Protein Dimer, His Tag

Product Category: Cytokine Receptor

Product Code: CSP-24015
Expression Host: HEK293T
Verified Applications: ELISA for IFNγR1-specific antibody and IFNγ cytokine ligand protein binding assays.
Suggested Applications: SPR & BLI for IFNγR1-specific antibody and IFNγ cytokine ligand protein binding assays. Animal immunization, RUO.
Purity: Greater than 90% dimer form as determined by SDS-PAGE under non-reducing condition

For Research Use Only (RUO)

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Formulation: 0.22μm filtered PBS, pH 7.4
Shipping: Frozen Dry Ice
Storage: -80’C

Datasheet

MSDS

Human IFNγR1 (interferon gamma receptor 1) is also known as CD119, IFNγR, IMD27A, and IMD27B. IFNγR1 protein dimer (CSP-24015) contains IFNγR1 2 extracellular domains (amino acids Glu18-Gly245, UniProt# P15260) with a homodimer motif and a His tag at the C-terminus. This protein is expressed in HEK293 cells. The IFNγR1 dimer protein is bioactive and can bind to IFNγ cytokine with dramatically increased binding potency compared to the IFNγR1 monomer. It also binds to IFNγR1-specific antibodies. This IFNγR1 dimer can be used as an immunogen for animal immunization (RUO) and as an antigen for in vitro assays and antibody screening targeting more conformational epitopes.
Product Name: IFNγ R1/CD119, Protein Dimer
UniProt #: P15260
Predicted Molecular Weight: 98 kDa
SDS PAGE Molecular Weight: The migration range of the dimer under non-reducing condition is 90-120 kDa on SDS PAGE.
Protein Construct: IFNγR1 protein dimer contains IFNγR1 2 extracellular domainswith a homodimer motif and a His tag at the C-terminus.

Background and Alternate Names

Interferon gamma receptor 1 (IFNγR1), also known as CD119, IMD27A and IMD27B, is a subunit of interferon gamma receptor (IFNγR). IFNγR belongs to the type II cytokine receptor family. IFNγR1 is a type I integral membrane glycoprotein containing extracellular, transmembrane and intracellular domains. The extracellular domain has two immunoglobulin-like (Ig-like) C2-type domains. The interferon gamma (IFNγ) dimer interacts with two IFNγR1 molecules to activate the cascade signaling pathway.

Alternate Names: CD119, IFNγR, IMD27A, and IMD27B