Immobilized human ADGRE2 fragment at 2 μg/mL (100 μL/well) can bind mouse Anti-Human ADGRE2 Monoclonal Antibody (Cat. No. CABh-25389) with half maximal effective concentration (EC50) range of 6.2-24.6 ng/mL (QC tested).
Detection of ADGRE2 expressed on HEK293 cell surface transiently transfected with full-length human ADGRE2 by Flow Cytometry. HEK293 cell line transfected with human ADGRE2 was stained with isotype control (black curve) or mouse Anti-Human ADGRE2 Monoclonal Antibody (Cat. No. CABh-25389) (green curve) by APC-conjugated Anti-Mouse IgG Secondary Antibody.

Anti-Human ADGRE2 Monoclonal Antibody

Product Category: Antibody

Product Code: CABh-25389
Isotype: IgG1
Specificity: Detects human adhesion G protein-coupled receptor E2/ADGRE2 transfectants but not irrelevant transfectants in flow cytometry.
Host Species: Mouse
Immunogen: ADGRE2 N-terminus, the exact sequence of the immunogen is proprietary.; UniProt # Q9UHX3
Purification: Affinity Enrichment
Expression Host: HEK293T
Verified Applications: ELISA, Flow cytometry
Recommended Usage: ELISA: starting concentration 1 µg/mL Flow cytometry: 0.8 µg/1E6 cells

For Research Use Only (RUO)

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Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4 with 10% trehalose as protectant
Shipping: Frozen Ice Packs
Storage: -20’C
Reconstitution Information:

For best performance, we strongly recommend you reconsitute the lyophilized product with deionized water to a stock solution of 500 µg/mL.
Solubilize for 20 minutes at room temperature with occasional gentle mixing. Avoid shaking or vortexing.

For 25µg product size:
Reconstitute with 50 µL sterile deionized water.

For 100µg product size:
Reconstitute with 200 µL sterile deionized water.

Datasheet

MSDS

Structure: 7-transmembrane protein, G protein-coupled receptor (GPCR)
Function: promotes cell-cell adhesion
Ligand/Receptor: chondroitin sulfate B
Cell Type: monocytes/macrophages, dendritic cells and all types of granulocytes
Molecular Family: G protein-coupled receptor
Gene ID: Q9UHX3
Product Name: ADGRE2, Monoclonal Antibody
UniProt #: Q9UHX3
Predicted Molecular Weight: N/A
Protein Construct: N/A

Background and Alternate Names

Human adhesion G protein-coupled receptor E2 (ADGRE2) is a 7-transmembrane G protein-coupled receptor (GPCR). ADGRE2 is a class B GPCR. ADGRE2 is mainly expressed on myeloid cells and binding to its ligand chondroitin sulfate B promotes cell attachment. High expression of ADGRE2 in acute myeloid leukemia is associated with poor patient outcomes. Human ADGRE2 protein has >95% sequence homology with mouse ADGRE2.

Alternate Names: Cluster of differentiation 312, CD312, VBU, EMR2, EGF-like module-containing mucin-like hormone receptor-like 2, EMR2