Immobilized human APLNR fragment at 2 μg/mL (100 μL/well) can bind mouse Anti-Human APLNR Monoclonal Antibody (Cat. No. CABh-25301) with half maximal effective concentration (EC50) range of 4.5-18.2 ng/mL (QC tested).
Detection of APLNR in HEK293 human cell line transiently transfected with human APLNR by Flow Cytometry. HEK293 cell line transfected with human APLNR was stained with isotype control (black curve) or Mouse Anti-Human APLNR Monoclonal Antibody (Cat. No. CABh-25301) (green curve) by APC-conjugated Anti-Mouse IgG Secondary Antibody.

Anti-Human APLNR Monoclonal Antibody

Product Category: All Products

Product Code: CABh-25301
Isotype: IgG2a
Specificity: Detects human Apelin receptor/APLNR transfectants but not irrelevant transfectants in flow cytometry.
Host Species: Mouse
Immunogen: APLNR N-terminus extracellular domain, the exact sequence of the immunogen is proprietary.; UniProt # P35414
Purification: Affinity Enrichment
Expression Host: HEK293T
Verified Applications: ELISA, Flow cytometry
Recommended Usage: ELISA: starting concentration 1 µg/mL Flow cytometry: 0.8 µg/1E6 cells

For Research Use Only (RUO)

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Formulation: 0.22μm filtered PBS, pH 7.4
Shipping: Frozen Dry Ice
Storage: -80’C

Datasheet

MSDS

Structure: 7-transmembrane protein, G protein-coupled receptor (GPCR)
Function: Cardiovascular, metabolic, and developmental processes
Ligand/Receptor: Apelin and ELABELA (also called Toddler)
Cell Type: Wide distribution, especially in the cardiovascular and endothelial systems
Molecular Family: G protein-coupled receptor
Gene ID: P35414
Product Name: APLNR, Monoclonal Antibody
UniProt #: P35414

Background and Alternate Names

Human Apelin receptor (APLNR), also known as APJ receptor, is a 7-transmembrane G protein-coupled receptor (GPCR). It is a Class A GPCR. APLNR plays a critical role in cardiovascular, metabolic, and developmental processes. It is activated by endogenous peptide ligands, primarily apelin and ELABELA (also called Toddler), which bind to the receptor and trigger intracellular signaling pathways. Dysregulation of the apelin–APLNR system has been linked to diseases such as heart failure, hypertension, diabetes, and obesity.

Alternate Names: AGTRL1, APJ, APJR, HG11