Immobilized human CX3CR1 fragment at 2 μg/mL (100 μL/well) can bind mouse Anti-Human CX3CR1 Monoclonal Antibody (Cat. No. CABh-25316) with half maximal effective concentration (EC50) range of 2.8-11 ng/mL (QC tested).
Immobilized human CX3CR1 VLP (Cat. No. CMP-25307) at 5 μg/mL (100 μL/well) can bind monoclonal anti-human CX3CR1 antibody (Cat. No. CABh-25316) with half maximal effective concentration (EC50) range of 0.7-2.8 μg/mL (QC tested).
Detection of CX3CR1 expressed on HEK293 cell surface transiently transfected with full-length human CX3CR1 by Flow Cytometry. HEK293 cell line transfected with human CX3CR1 was stained with isotype control (black curve) or mouse Anti-Human CX3CR1 Monoclonal Antibody (Cat. No. CABh-25316) (green curve) by APC-conjugated Anti-Mouse IgG Secondary Antibody.

Anti-Human CX3CR1 Monoclonal Antibody

Product Category: All Products

Product Code: CABh-25316
Isotype: IgG2a
Specificity: Detects human CX3C motif chemokine receptor 1/CX3CR1 transfectants but not irrelevant transfectants in flow cytometry.
Host Species: Mouse
Immunogen: CX3CR1 N-terminus, the exact sequence of the immunogen is proprietary.; UniProt # P49238
Purification: Affinity Enrichment
Expression Host: HEK293T
Verified Applications: ELISA, Flow cytometry
Recommended Usage: ELISA: starting concentration 1 µg/mL Flow cytometry: 0.8 µg/1E6 cells

For Research Use Only (RUO)

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Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4 with 10% trehalose as protectant
Shipping: Frozen Ice Packs
Storage: -20’C

Datasheet

MSDS

Structure: 7-transmembrane protein, G protein-coupled receptor (GPCR)
Function: mediates recruitment of immune cells
Ligand/Receptor: CX3CL1
Cell Type: numerous cell types, including T lymphocytes, natural killer cells, and macrophages
Molecular Family: G protein-coupled receptor
Gene ID: P49238
Product Name: CX3CR1, Monoclonal Antibody
UniProt #: P49238
Predicted Molecular Weight: N/A
Protein Construct: N/A

Background and Alternate Names

Human CX3C motif chemokine receptor 1 (CX3CR1), is a 7-transmembrane G protein-coupled receptor (GPCR). CX3CR1 is a class A GPCR. CX3CR1 is expressed on numerous cell types, including various cells from hematopoietic lineage such as T lymphocytes, natural killer cells, and macrophages. Interaction of CX3CR1 with its ligand CX3CL1 mediates recruitment of immune cells by migration through chemotaxis and diapedesis as well as mediating migration, adhesion and retention of leukocytes. CX3CR1 increases accumulation of immune cells in affected areas of the body, which can result in aggravation of several diseases such as Rheumatoid arthritis, Renal diseases, Chronic liver disease, and Crohn’s disease. CX3CR1 is also a coreceptor for HIV-1/HIV-2 and respiratory syncytial virus (RSV). In the tumor microenvironment (TME), CX3CR1 plays a dual role depending on context — it can promote immune cell infiltration and anti-tumor activity, or contribute to immune suppression and poor prognosis. Human CX3CR1 protein has ~99% sequence homology with mouse CX3CR1.

Alternate Names: Fractalkine receptor, G-protein coupled receptor 13, GPR13, CCRL1, CMKBRL1, CMKDR1, GPRV28, V28, C-X3-C motif chemokine receptor 1