Immobilized human EDNRB fragment at 2 μg/mL (100 μL/well) can bind mouse Anti-Human EDNRB Monoclonal Antibody (Cat. No. CABh-25398) with half maximal effective concentration (EC50) range of 6.3-25.4 ng/mL (QC tested).
Detection of EDNRB expressed on HEK293 cell surface transiently transfected with full-length human EDNRB by Flow Cytometry. HEK293 cell line transfected with human EDNRB was stained with isotype control (black curve) or mouse Anti-Human EDNRB Monoclonal Antibody (Cat. No. CABh-25398) (green curve) by APC-conjugated Anti-Mouse IgG Secondary Antibody.

Anti-Human EDNRB Monoclonal Antibody

Product Category: Antibody

Product Code: CABh-25398
Isotype: IgG2a
Specificity: Detects human endothelin receptor type B/EDNRB transfectants but not irrelevant transfectants in flow cytometry.
Host Species: Mouse
Immunogen: EDNRB N-terminus, the exact sequence of the immunogen is proprietary.; UniProt # P24530
Purification: Affinity Enrichment
Expression Host: HEK293T
Verified Applications: ELISA, Flow cytometry
Recommended Usage: ELISA: starting concentration 1 µg/mL Flow cytometry: 0.8 µg/1E6 cells

For Research Use Only (RUO)

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Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4 with 10% trehalose as protectant
Shipping: Frozen Ice Packs
Storage: -20’C
Reconstitution Information:

For best performance, we strongly recommend you reconsitute the lyophilized product with deionized water to a stock solution of 500 µg/mL.
Solubilize for 20 minutes at room temperature with occasional gentle mixing. Avoid shaking or vortexing.

For 25µg product size:
Reconstitute with 50 µL sterile deionized water.

For 100µg product size:
Reconstitute with 200 µL sterile deionized water.

Datasheet

MSDS

Structure: 7-transmembrane protein, G protein-coupled receptor (GPCR)
Function: activates phosphatidylinositol-calcium second messenger system
Ligand/Receptor: endothelins
Cell Type: various tissues, especially placental cells
Molecular Family: G protein-coupled receptor
Gene ID: P24530
Product Name: EDNRB, Monoclonal Antibody
UniProt #: P24530
Predicted Molecular Weight: N/A
Protein Construct: N/A

Background and Alternate Names

Human endothelin receptor type B (EDNRB) is a 7-transmembrane G protein-coupled receptor (GPCR). EDNRB is a class A GPCR. EDNRB interacts with endothelins (ET-1, ET-2, ET-3), vasoactive peptides involved in cell proliferation, migration, and immune regulation. It can regulate critical biological processes such as the development and function of blood vessels, hormone production, and the stimulation of cell growth and proliferation. In cancer, EDNRB expression is often dysregulated and can act as a tumor suppressor or, in some contexts, contribute to tumor progression. EDNRB has been found to be a prognostic marker in kidney renal clear cell carcinoma and lung squamous cell carcinoma. Human EDNRB protein has >80% sequence homology with mouse EDNRB.

Alternate Names: ABCDS, ET-B, ET-BR, ETB, ETBR, ETRB, HSCR, HSCR2, WS4A, ETB1