Detection of FZD10 in HEK293 human cell line transiently transfected with human FZD10 by Flow Cytometry. HEK293 cell line transfected with human FZD10 was stained with isotype control (black curve) or Mouse Anti-Human FZD10 Monoclonal Antibody (Cat. No. CABh-25330) (green curve) by APC-conjugated Anti-Mouse IgG Secondary Antibody.
Immobilized human FZD10 peptide can bind Mouse Anti-Human FZD10 Monoclonal Antibody (Cat. No. CABh-25330) with a KD of 10.1-40.4 nM as determined by LSPR (Nicoya Alto).

Anti-Human FZD10 Monoclonal Antibody

Antibody

Product Code: CABh-25330
Isotype: IgG2a
Conjugate: Unconjugated
Specificity: Detects human frizzled class receptor 10/FZD10 transfectants but not irrelevant transfectants in flow cytometry.
Host Species: Mouse
Immunogen: FZD10 N-terminus, the exact sequence of the immunogen is proprietary.; UniProt # Q9ULW2
Purification: Affinity Enrichment
Expression Host: HEK293T
Verified Applications: ELISA, Flow cytometry, SPR
Recommended Usage: ELISA: starting concentration 1 µg/mL Flow cytometry: 0.2-0.8 µg/1E6 cells LSPR: Recommended maximum concentration, 100 nM

For Research Use Only (RUO)

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Formulation: 0.22μm filtered PBS, pH 7.4
Shipping: Frozen Dry Ice
Storage: -80’C

Datasheet

MSDS

Structure: 7-transmembrane protein, G protein-coupled receptor (GPCR)
Function: Wnt/beta-catenin canonical pathway signaling
Ligand/Receptor: Wnt proteins
Cell Type: Variable expression in most tissues; overexpressed in multiple cancer cells, for example, Synovial sarcoma, colorectal cancer, gastric cancer
Molecular Family: G protein-coupled receptor
Gene ID: Q9ULW2
Product Name: Anti-huFZD10
Human frizzled class receptor 10 (FZD10), also called Frizzled-10 (Fz-10), Fz10, FzE7, hFz10, or cluster of differentiation 350 (CD350), is a 7-transmembrane G protein-coupled receptor (GPCR). FZD10 is a member of the frizzled gene family, Class F GPCR family. The most frizzled receptors are coupled to the Wnt/beta-catenin canonical signaling pathway. FZD10 is a receptor for Wnt proteins, mediating Wnt-dependent signal transduction. FZD10 is implicated in many types of cancer and is specifically a therapeutic target for colorectal cancer as it is significantly up-regulated in some cases of primary colon cancer. Human FZD10 protein has ~99% and ~93% sequence homology with macaque and mouse FZD10, respectively.

Alternate Names: Frizzled-10, Fz-10, Fz10, FzE7, hFz10, cluster of differentiation 350, CD350