Immobilized human GPR56 fragment at 2 μg/mL (100 μL/well) can bind mouse Anti-Human GPR56 Monoclonal Antibody (Cat. No. CABh-24116) with half maximal effective concentration (EC50) range of 6-24 ng/mL (QC tested).
Detection of GPR56 expressed on HEK293 cell surface transiently transfected with full-length human GPR56 by Flow Cytometry. HEK293 cell line transfected with human GPR56 was stained with isotype control (black curve) or mouse Anti-Human GPR56 Monoclonal Antibody (Cat. No. CABh-24116) (green curve) by APC-conjugated Anti-Mouse IgG Secondary Antibody.

Anti-Human GPR56 Monoclonal Antibody

Product Code: CABh-24116
Isotype: IgG2a
Specificity: Detects human G protein-coupled receptor 56/GPR56 transfectants but not irrelevant transfectants in flow cytometry.
Host Species: Mouse
Immunogen: GPR56 N-terminus, the exact sequence of the immunogen is proprietary.; UniProt # Q9Y653
Purification: Affinity Enrichment
Verified Applications: ELISA, Flow cytometry
Recommended Usage: ELISA: starting concentration 1 µg/mL Flow cytometry: 0.8 µg/1E6 cells

For Research Use Only (RUO)

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Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4 with 10% trehalose as protectant
Shipping: Frozen Ice Packs
Storage: -20’C
Reconstitution Information:

For best performance, we strongly recommend you reconsitute the lyophilized product with deionized water to a stock solution of 500 µg/mL.
Solubilize for 20 minutes at room temperature with occasional gentle mixing. Avoid shaking or vortexing.

For 25µg product size:
Reconstitute with 50 µL sterile deionized water.

For 100µg product size:
Reconstitute with 200 µL sterile deionized water.

Datasheet

MSDS

Structure: 7-transmembrane protein, G protein-coupled receptor (GPCR)
Function: hematopoietic cluster formation
Ligand/Receptor: transglutaminase 2 and collagen III
Cell Type: neural progenitor cells, melanoma cells, and lymphocytes, such as effector memory T cells, γδ T cells, and NK cells
Molecular Family: G protein-coupled receptor
Gene ID: Q9Y653
Product Name: GPR56, Monoclonal Antibody
Human G protein-coupled receptor 56 (GPR56) is a 7-transmembrane G protein-coupled receptor (GPCR), also known as TM7XN1, encoded by the ADGRG1 gene. GPR56 is an orphan GPCR. GPR56 is required for hematopoietic cluster formation during endothelial to hematopoietic stem cell transition. Down-regulation of GPR56 has been linked to severe depression and overexpression of GPR56 has been detected in many cancers such as breast, colon, lung, and ovarian making GPR56 a possible therapeutic target. Human GPR56 protein has >75% sequence homology with mouse GPR56.

Alternate Names: ADGRG1, BFPP, BPPR, TM7LN4, TM7XN1, adhesion G protein-coupled receptor G1