Immobilized human LGR4 at 2 μg/mL (100 μL/well) can bind Mouse Anti-Human LGR4 Monoclonal Antibody (Cat. No. CABh-25292) with half maximal effective concentration (EC50) range of 19.6-78.2 ng/mL (QC tested).
Immobilized human LGR4 protein can bind Mouse Anti-Human LGR4 Monoclonal Antibody (Cat. No. CABh-25292) with a KD of 0.1-0.4 nM as determined by LSPR (Nicoya Alto).

Anti-Human LGR4 Monoclonal Antibody

Product Category: Antibody

Product Code: CABh-25292
Isotype: IgG2a
Conjugate: Unconjugated
Specificity: Human Leucine-rich repeat-containing G-protein coupled receptor 4/LGR4 shows low affinity binding to LGR4 transfected cells by flow cytometry.
Host Species: Mouse
Immunogen: LGR4 N-terminus, the exact sequence of the immunogen is proprietary.; UniProt # Q9BXB1
Purification: Affinity Enrichment
Expression Host: HEK293T
Verified Applications: ELISA, SPR
Recommended Usage: ELISA: starting concentration 1 µg/mL. LSPR: Recommended maximum concentration, 100 nM.

For Research Use Only (RUO)

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Formulation: 0.22μm filtered PBS, pH 7.4
Shipping: Frozen Dry Ice
Storage: -80’C

Datasheet

MSDS

Structure: 7-transmembrane protein, G protein-coupled receptor (GPCR)
Function: Activator of the Wnt signaling pathway required for the development of various organs
Ligand/Receptor: R-spondins
Cell Type: Variable expression in most tissues; over-expressed on cancer cells, for example, lung cancer adenocarcinomas
Molecular Family: G protein-coupled receptor
Gene ID: Q9BXB1
Product Name: Anti-huLGR4
UniProt #: Q9BXB1
Predicted Molecular Weight: N/A
Protein Construct: N/A

Background and Alternate Names

Human Leucine-rich repeat-containing G-protein coupled receptor 4 (LGR4), also called BNMD17, GPR48, and DPSL, is a 7-transmembrane G protein-coupled receptor (GPCR). It is a Class B GPCR. Upon binding R-spondins, LGR4 triggers the canonical Wnt signaling pathway to increase expression of target genes. Unlike most classical G-protein coupled receptors, LGR4 does not activate heterotrimeric G-proteins to transduce the signal. LGR4 is a potential diagnostic biomarker and therapeutic target due to its promotion of tumorigenesis in some cancers. Human LGR4 protein has ~97% and ~93% sequence homology with macaque and mouse LGR4, respectively.

Alternate Names: BNMD17, GPR48, DPSL