Immobilized human MC5R fragment at 2 μg/mL (100 μL/well) can bind Mouse Anti-Human MC5R Monoclonal Antibody (Cat. No. CABh-25407) with half maximal effective concentration (EC50) range of 8.8-35.2 ng/mL (QC tested).
Detection of MC5R expressed on HEK293 cell surface transiently transfected with full-length human MC5R by Flow Cytometry. HEK293 cell line transfected with human MC5R was stained with isotype control (black curve) or mouse Anti-Human MC5R Monoclonal Antibody (Cat. No. CABh-25407) (green curve) by APC-conjugated Anti-Mouse IgG Secondary Antibody.

Anti-Human MC5R Monoclonal Antibody

Product Category: All Products

Product Code: CABh-25407
Isotype: IgG2a
Specificity: Detects human melanocortin 5 receptor / MC5R transfectants but not irrelevant transfectants in flow cytometry.
Host Species: Mouse
Immunogen: MC5R N-terminus, the exact sequence of the immunogen is proprietary.; UniProt # P33032
Purification: Affinity Enrichment
Expression Host: HEK293T
Verified Applications: ELISA, Flow cytometry
Recommended Usage: ELISA: starting concentration 1 µg/mL Flow cytometry: 0.8 µg/1E6 cells

For Research Use Only (RUO)

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Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4 with 10% trehalose as a protectant
Shipping: Frozen Ice Packs
Storage: On receipt, store at -20 C or lower for long term storage. This product is stable at: -20 to -70 C for 12 months from date of receipt in lyophilized state. -70 C for 3 months after reconstitution under sterile conditions. Avoid repeated freeze-thaw cycles
Reconstitution Information:

For best performance, we strongly recommend you reconsitute the lyophilized product with deionized water to a stock solution of 500 µg/mL.
Solubilize for 20 minutes at room temperature with occasional gentle mixing. Avoid shaking or vortexing.

For 25µg product size:
Reconstitute with 50 µL sterile deionized water.

For 100µg product size:
Reconstitute with 200 µL sterile deionized water.

Datasheet

MSDS

Structure: 7-transmembrane protein, G protein-coupled receptor (GPCR)
Function: regulation of sebaceous lipids
Ligand/Receptor: melanocyte-stimulating hormone (MSH) and adrenocorticotrophin hormone (ACTH)
Cell Type: Several cell types including sebocytes and epithelial cells
Molecular Family: G protein-coupled receptor
Gene ID: P33032
Product Name: MC5R, Monoclonal Antibody
UniProt #: P33032
Predicted Molecular Weight: N/A
Protein Construct: N/A

Background and Alternate Names

Human melanocortin 5 receptor (MC5R) is a 7-transmembrane G protein-coupled receptor (GPCR). MC5R is a class A GPCR. MC5R is highly expressed in several cell types including sebocytes and epithelial cells. MC5R has been shown to be important in the regulation of sebum production. Stimulation of MC5R promotes fatty acid oxidation in skeletal muscle and lipolysis in adipocytes. It is essential for erythrocyte differentiation and involved in inflammation. Due to its involvement in sebogenesis, MC5R has become an emerging therapeutic target for sebum-suppressive therapies for seborrhoea and acne vulgaris. Human MC5R protein has >80% sequence homology with mouse MC5R.

Alternate Names: MC2