MW: Molecular Weight marker reduced condition NR:

Immobilize human IL10Rα dimer protein (Cat. No. CSP-24018) at 2 μg/mL (100 μL/well) can bind anti-human IL10Rα monoclonal antibody with half maximal effective concentration (EC50) range of 14.6-58.2 ng/mL (QC tested).

Immobilize human IL10 protein at 2 μg/mL (100 μL/well) can bind IL10Rα dimer protein (Cat. No. CSP-24018) with half maximal effective concentration (EC50) range of 3.4 - 13.5 ng/mL (QC tested).

Bioactive, Human IL-10Rα Protein Dimer, His Tag

Cytokine Receptor

Product Code: CSP-24018

Expression Host: HEK293T

Verified Applications: ELISA for IL10Rα-specific antibody and IL10 cytokine ligand protein binding assays.

Suggested Applications: SPR & BLI for IL10Rα-specific antibody and IL10 cytokine ligand protein binding assays.

Purity: Greater than 90% dimer form as determined by SDS-PAGE under non-reducing condition

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Formulation: 0.22μm filtered PBS, pH 7.4

Shipping: Frozen Dry Ice

Storage: -80’C

Datasheet

MSDS

Human IL10Rα (interleukin 10 receptor subunit alpha) is also known as CD210, IL10R, CD210α, CDW210A, and IL-10R1. IL10Rα protein dimer (CSP-24018) contains 2 IL10Rα extracellular domains (amino acids His22-Asn235, UniProt# Q13651) with a homodimer motif and a His tag at the C-terminus. This protein is expressed in HEK293 cells. The IL10Rα dimer protein is bioactive and can bind to IL10 cytokine with dramatically increased binding potency compared with the IL10Rα monomer. It also binds to IL10Rα-specific antibodies. This IL10Rα dimer can be used as an immunogen for animal immunization (RUO) and as an antigen for in vitro assays and antibody screening targeting more conformational epitopes.

Protein Name: IL-10Rα

UniProt #: Q13651

Predicted Molecular Weight: 101 kDa

SDS PAGE Molecular Weight: The migration range of the dimer under non-reducing condition is 100-150kDa on SDS PAGE.

Protein Construct: IL10Rα protein dimer contains 2 extracellular domainswith a homodimer motif and a His tag at the C-terminus.

Background

Interleukin-10 receptor alpha (IL10Rα), also known as IL-10 R1, CD210, CD210α, CDW210A, is a subunit of interleukin-10 receptor (IL10R) complex. IL10R belongs to the type II cytokine receptor family. IL10Rα is a type I integral membrane glycoprotein containing extracellular, transmembrane and intracellular domains. The extracellular domain has two immunoglobulin-like (Ig-like) C2-type domains. IL-10 cytokine dimer interacts with two IL10Rα molecules to activate the cascade signaling pathway.

Bioactive, Human IL-10Rα Protein Dimer, His Tag

Cytokine Receptor

Datasheet

MSDS

Background

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