MW: Molecular Weight marker reduced condition NR: CD8α dimer under non-reduced condition
Immobilized human CD8α protein dimer, His tag (Cat. No. CSP-25135-01) can bind anti-human CD8α polyclonal antibody with half maximal effective concentration (EC50) range of 6.8-27.3 ng/mL (QC tested).
Immobilized human PILRα can bind human CD8α protein dimer, His tag (Cat. No. CSP-25135-01) with a KD of 6.8-27.4 nM as determined by LSPR (Nicoya Alto).

Bioactive, Recombinant Human CD8 alpha Protein Dimer, His Tag

Product Category: T-cell Receptor

Product Code: CSP-25135-01
Expression Host: HEK293T
Verified Applications: ELISA for CD8α-specific antibody binding assays and SPR for PILRα ligand protein binding assays.
Suggested Applications: SPR & BLI for CD8α-specific antibody and BLI for PILRα protein binding assays. Animal immunization, RUO.
Purity: Greater than 90% dimer form as determined by SDS-PAGE under non-reducing condition
Amino Acid Range: S22-D182

For Research Use Only (RUO)

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Formulation: 0.22μm filtered PBS, pH 7.4
Shipping: Frozen Dry Ice
Storage: -80’C

Datasheet

MSDS

Cluster of differentiation 8 alpha (CD8α, CD8a), also known as T-cell surface glycoprotein CD8 alpha chain, is a Type I transmembrane glycoprotein and is a component of the CD8 coreceptor. The recombinant CD8α dimer protein (CSP-25135-01) is a cis-homodimer (cis-dimer) and contains the CD8α extracellular domain (UniProt# 1P01732, amino acids Ser22-Asp182) fused with a proprietary cis-dimer motif followed by a His tag at the C-terminus. This dimeric protein is expressed in HEK293T cells. The recombinant human CD8α dimer protein is bioactive and can bind to PILR-alpha that acts as a ligand for CD8α. It also binds CD8α-specific antibodies. This CD8α dimer can be used as an antigen for in vitro assays and antibody screening, and as an immunogen for immunization to generate antibodies targeting more conformational epitopes.
Product Name: CD8a
UniProt #: P01732
Predicted Molecular Weight: 51 kDa
SDS PAGE Molecular Weight: The migration range of the heterodimer protein with glycosylation under non-reducing condition is between 60 and 85 kDa on SDS PAGE.
Protein Construct: CD8α homodimer protein contains the CD8α extracellular domain (UniProt# 1P01732) fused with a proprietary cis-dimer motif followed by a His tag at the C-terminus.

Background and Alternate Names

Cluster of differentiation 8 alpha (CD8α, CD8a), also known as T-cell surface glycoprotein CD8 alpha chain, is a Type I transmembrane glycoprotein and is a component of the CD8 coreceptor. CD8 is expressed on the T-cell surface as dimers in two isoforms, the CD8αα (CD8 alpha-alpha) homodimer and the CD8αβ (CD8 alpha-beta) heterodimer. CD8 is a key component of T cells, contributing to antigen recognition, immune cell maturation, and immune cell signaling. CD8 acts as the co-receptor for the T-cell receptor (TCR) and binds a major histocompatibility complex (MHC) molecule. CD8α is predominantly expressed on the surface of cytotoxic T cells and contains an extracellular domain with a single immunoglobulin variable (IgV)-like domain and a proline-rich stalk region. PILRα acts as a ligand for CD8α and their interaction plays a crucial role in maintaining CD8+ T cell quiescence. CD8 is highly expressed in T cell lymphoblastic lymphoma and hypo-pigmented mycosis fungoides making it an emerging target of cancer therapeutics.

Alternate Names: T-cell surface glycoprotein CD8 alpha chain, CD8α, CD8, Leu2, MAL