MW: Molecular Weight marker reduced condition NR: CD8α dimer under non-reduced condition

Immobilized human CD8α protein dimer

His tag (Cat. No. CSP-25135-01) can bind anti-human CD8α polyclonal antibody with half maximal effective concentration (EC50) range of 6.8-27.3 ng/mL (QC tested).

Bioactive, Recombinant Human CD8 alpha Protein Dimer, His Tag

T-cell Receptor

Product Code: CSP-25135-01

Expression Host: HEK293T

Verified Applications: ELISA for CD8α-specific antibody binding assays and SPR for PILRα ligand protein binding assays.

Suggested Applications: SPR & BLI for CD8α-specific antibody and BLI for PILRα protein binding assays. Animal immunization, RUO.

Purity: Greater than 90% dimer form as determined by SDS-PAGE under non-reducing condition

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Formulation: 0.22μm filtered PBS, pH 7.4

Shipping: Frozen Dry Ice

Storage: -80’C

Cluster of differentiation 8 alpha (CD8α, CD8a), also known as T-cell surface glycoprotein CD8 alpha chain, is a Type I transmembrane glycoprotein and is a component of the CD8 coreceptor. The recombinant CD8α dimer protein (CSP-25135-01) is a cis-homodimer (cis-dimer) and contains the CD8α extracellular domain (UniProt# 1P01732, amino acids Ser22-Asp182) fused with a proprietary cis-dimer motif followed by a His tag at the C-terminus. This dimeric protein is expressed in HEK293T cells. The recombinant human CD8α dimer protein is bioactive and can bind to PILR-alpha that acts as a ligand for CD8α. It also binds CD8α-specific antibodies. This CD8α dimer can be used as an antigen for in vitro assays and antibody screening, and as an immunogen for immunization to generate antibodies targeting more conformational epitopes.

Protein Name: CD8a

UniProt #: 1P01732

Predicted Molecular Weight: 51 kDa

SDS PAGE Molecular Weight: The migration range of the heterodimer protein with glycosylation under non-reducing condition is between 60 and 85 kDa on SDS PAGE.

Protein Construct: CD8α homodimer protein contains the CD8α extracellular domainfused with a proprietary cis-dimer motif followed by a His tag at the C-terminus.

Background

Cluster of differentiation 8 alpha (CD8α, CD8a), also known as T-cell surface glycoprotein CD8 alpha chain, is a Type I transmembrane glycoprotein and is a component of the CD8 coreceptor. CD8 is expressed on the T-cell surface as dimers in two isoforms, the CD8αα (CD8 alpha-alpha) homodimer and the CD8αβ (CD8 alpha-beta) heterodimer. CD8 is a key component of T cells, contributing to antigen recognition, immune cell maturation, and immune cell signaling. CD8 acts as the co-receptor for the T-cell receptor (TCR) and binds a major histocompatibility complex (MHC) molecule. CD8α is predominantly expressed on the surface of cytotoxic T cells and contains an extracellular domain with a single immunoglobulin variable (IgV)-like domain and a proline-rich stalk region. PILRα acts as a ligand for CD8α and their interaction plays a crucial role in maintaining CD8+ T cell quiescence. CD8 is highly expressed in T cell lymphoblastic lymphoma and hypo-pigmented mycosis fungoides making it an emerging target of cancer therapeutics.

Bioactive, Recombinant Human CD8 alpha Protein Dimer, His Tag

T-cell Receptor

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