MW: Molecular Weight marker reduced condition NR: CD80 dimer under non-reduced condition
Immobilized mouse CD80 protein dimer, His Tag (CSP-25187-02) at 2 μg/mL (100 μL/well) can bind anti-mouse CD80 polyclonal antibody with half maximal effective concentration (EC50) range of 4.7-18.9 ng/mL (QC tested).
Immobilized mouse CTLA-4 protein dimer, His Tag (CSP-25185-01) at 2 μg/mL (100 μL/well) can bind mouse CD80 protein dimer, His Tag (CSP-25187-02) with half maximal effective concentration (EC50) range of 6.8-27.4 ng/mL (QC tested).
Immobilized mouse CTLA-4 protein dimer, His tag (Cat. No. CSP-25185-01) can bind mouse CD80 protein dimer, FLAG-His tag (Cat. No. CSP-25187-02) with a KD of 2.5-9.9 nM as determined by LSPR (Nicoya Alto).

Bioactive, Recombinant Mouse CD80 Protein Dimer, FLAG-His Tag

Product Category: Immune Checkpoint

Product Code: CSP-25187-02
Expression Host: HEK293T
Verified Applications: ELISA for CD80-specific antibody binding assays and SPR and ELISA for CD80 and CTLA-4 ligand protein binding assays.
Suggested Applications: SPR & BLI for CD80-specific antibody binding assays and BLI for CD80 and CTLA-4 or CD28 protein binding assays. Animal immunization, RUO.
Purity: Greater than 90% dimer form as determined by SDS-PAGE under non-reducing condition
Amino Acid Range: V38-N246

For Research Use Only (RUO)

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Formulation: 0.22μm filtered PBS, pH 7.4
Shipping: Frozen Dry Ice
Storage: -80’C

Datasheet

MSDS

Cluster of differentiation 80 (CD80) is a Type I transmembrane glycoprotein in the immunoglobulin superfamily and a member of the B7 Family of ligands. CD80 exists as a monomer but its dimeric form can influence immune regulation and contribute to pathogenic conditions. The recombinant CD80 dimer protein (CSP-25187-02) is a cis-homodimer (cis-dimer) and contains a CD80 extracellular domain (UniProt# Q00609, amino acids Val38-Asn246) fused with a dimer motif followed by a tandem FLAG-His tag at the C-terminus. This dimeric protein is expressed in HEK293T cells. The recombinant CD80 dimer protein is bioactive and can bind to CTLA-4 and CD28. It also binds CD80-specific antibodies. This CD80 dimer can be used as an antigen for in vitro assays and antibody screening, and an immunogen for immunization to generate antibodies targeting more conformational epitopes.
Product Name: CD80, Protein Dimer
UniProt #: Q00609
Predicted Molecular Weight: 66 kDa
SDS PAGE Molecular Weight: The migration range of the heterodimer protein with glycosylation under non-reducing condition is between 120 and 190 kDa on SDS PAGE.
Protein Construct: CD80 dimer protein contains the CD80 extracellular domainfused with a proprietary dimer motif followed by a tandem FLAG-His tag at the C-terminus.

Background and Alternate Names

Cluster of differentiation 80 (CD80) is a Type I transmembrane glycoprotein in the immunoglobulin superfamily and a member of the B7 Family of ligands. CD80 is also known as B7, B7-1, B7.1, BB1, CD28LG, CD28LG1, and LAB7. CD80 contains an extracellular domain (ECD), a transmembrane domain, and a cytoplasmic domain. The ECD consists of two immunoglobulin (Ig)-like subdomains, a variable-like domain (Ig-V-like domain), and a constant-like domain (Ig-C-like domain). It is primarily expressed on antigen-presenting cells (APCs), such as dendritic cells, macrophages, and B cells. CD80 interacts with CTLA-4 (Cytotoxic T-lymphocyte associated protein 4) to transmit an inhibitory signal with T cells and CD28 (Cluster of differentiation 28) to transmit a stimulatory signal. It is often overexpressed in various autoimmune diseases such as multiple sclerosis and systemic lupus erythematosus, as well as some cancers. CD80 exists as a monomer but its dimeric form can influence immune regulation and contribute to pathogenic conditions. A recombinant protein mimicking the CD80 dimer conformation can be crucial for therapeutic discovery. While structurally and functionally similar to human CD80, mouse CD80 is a species-specific tool essential for preclinical studies, basic research and translational research in cancer immunotherapy.

Alternate Names: B7, B7-1, B7.1, BB1, CD28LG, CD28LG1, LAB7