MW: Molecular Weight marker reduced conditionNR: CD80 dimer under non-reduced condition
Immobilized Rhesus macaque CD80 protein dimer, His Tag (CSP-25293-01) at 2 μg/mL (100 μL/well) can bind anti-human CD80 monoclonal antibody with half maximal effective concentration (EC50) range of 0.9-3.5 ng/mL (QC tested).
Immobilized Rhesus macaque CD80 protein dimer, His Tag (CSP-25293-01) at 2 μg/mL (100 μL/well) can bind Rhesus macaque CTLA-4 with half maximal effective concentration (EC50) range of 0.7-2.7 ng/mL (QC tested).
Immobilized Rhesus macaque CTLA-4 can bind Rhesus macaque CD80 protein dimer, His Tag (CSP-25293-01) with a KD of 1.3-5.3 nM as determined by LSPR (Nicoya Alto).

Bioactive, Recombinant Rhesus macaque CD80 Protein Dimer, His Tag

Product Category: Immune checkpoint

Product Code: CSP-25293-01
Expression Host: HEK293T
Verified Applications: SPR and ELISA for CTLA-4 ligand protein binding assays. ELISA for CD80-specific antibody binding assays.
Suggested Applications: SPR & BLI for CD80-specific antibody binding assays and BLI for CTLA-4 protein binding assays. Animal immunization, RUO.
Purity: Greater than 90% dimer form as determined by SDS-PAGE under non-reducing condition
Amino Acid Range: V35-N242

For Research Use Only (RUO)

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Formulation: 0.22μm filtered PBS, pH 7.4
Shipping: Frozen Dry Ice
Storage: -80’C

Datasheet

MSDS

CD80 (Cluster of differentiation 80) is a type I transmembrane glycoprotein in the immunoglobulin superfamily and a member of the B7 Family of ligands. CD80 exists as a monomer but its dimeric form can influence immune regulation and contribute to pathogenic conditions. The recombinant CD80 dimer protein (CSP-25293-01) is a cis-homodimer (cis-dimer) and contains a CD80 extracellular domain (UniProt# G7NXN7, amino acids Val35-Asn242) fused with a dimer motif followed by a His tag at the C-terminus. This dimeric protein is expressed in HEK293T cells. The recombinant CD80 dimer protein is bioactive and can bind to CTLA-4 or CD28. It also binds CD80-specific antibodies. This CD80 dimer can be used as an antigen for in vitro assays and antibody screening, and an immunogen for immunization to generate antibodies targeting more conformational epitopes.
Product Name: CD80
UniProt #: G7NXN7
Predicted Molecular Weight: 64 kDa
SDS PAGE Molecular Weight: The migration range of the heterodimer protein with glycosylation under non-reducing condition is 85 to 120 kDa on SDS PAGE.
Protein Construct: CD80 dimer protein contains a CD80 extracellular domainfused with a proprietary dimer motif followed by a His tag at the C-terminus.

Background and Alternate Names

Rhesus macaque CD80 (Cluster of differentiation 80) is a type I transmembrane glycoprotein in the immunoglobulin superfamily and a member of the B7 Family of ligands. CD80 is also known as B7, B7-1, B7.1, BB1, CD28LG, CD28LG1, and LAB7. CD80 contains an extracellular domain consisting of two immunoglobulin (Ig)-like subdomains, a variable-like domain (Ig-V-like domain), and a constant-like domain (Ig-C-like domain). It is primarily expressed on antigen-presenting cells (APCs), such as dendritic cells, macrophages, and B cells. CD80 interacts with CTLA-4 (Cytotoxic T-lymphocyte associated protein 4) to transmit an inhibitory signal with T cells and CD28 (Cluster of differentiation 28) to transmit a stimulatory signal. It is often overexpressed in various autoimmune diseases such as multiple sclerosis and systemic lupus erythematosus, as well as some cancers. CD80 exists as a monomer but its dimeric form can influence immune regulation and contribute to pathogenic conditions. A recombinant protein mimicking the CD80 dimer conformation can be crucial for therapeutic discovery. While structurally and functionally similar to human CD80 homodimer, Rhesus macaque CD80 homodimer is a species-specific tool essential for preclinical studies, basic research, and translational research.

Alternate Names: B7, B7-1, B7.1, BB1, CD28LG, CD28LG1, LAB7