MW: Molecular Weight marker reduced condition NR: CD274 dimer under non-reduced condition
Immobilized Rhesus macaque CD274 protein dimer, His Tag (CSP-25292-01) at 2 μg/mL (100 μL/well) can bind anti-human CD274 polyclonal antibody with half maximal effective concentration (EC50) range of 5.3-21.2 ng/mL (QC tested).
Immobilized Rhesus macaque CD274 protein dimer, His Tag (CSP-25292-01) at 2 μg/mL (100 μL/well) can human PD1 with half maximal effective concentration (EC50) range of 0.2-0.9 μg/mL (QC tested).
Immobilized human PD1 can bind Rhesus macaque CD274 protein dimer, His Tag (CSP-25292-01) with a KD of 5.5-21.8 nM as determined by LSPR (Nicoya Alto).

Bioactive, Rhesus macaque CD274 Protein Dimer, His Tag

Product Category: Immune Checkpoint

Product Code: CSP-25292-01
Expression Host: HEK293T
Verified Applications: ELISA for CD274-specific antibody binding assays and ELISA and SPR for PD-1 ligand protein binding assays.
Suggested Applications: SPR & BLI for CD274-specific antibody binding assays and BLI for PD-1 protein binding assays. Animal immunization, RUO.
Purity: Greater than 90% dimer form as determined by SDS-PAGE under non-reducing condition
Amino Acid Range: F19-R238

For Research Use Only (RUO)

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Formulation: 0.22μm filtered PBS, pH 7.4
Shipping: Frozen Dry Ice
Storage: -80’C

Datasheet

MSDS

Rhesus macaque cluster of differentiation 274 (CD274), is a type I membrane protein in the immunoglobulin superfamily and a member of the B7 Family of ligands. The recombinant CD274 dimer protein (CSP-25292-01) is a cis-homodimer (cis-dimer) and contains a CD274 extracellular domain (UniProt# G7PSE7, amino acids Phe19-Arg238) fused with a proprietary dimer motif followed by a His tag at the C-terminus. This dimeric protein is expressed in HEK293T cells. The recombinant Rhesus macaque CD274 dimer protein is bioactive and can bind to PD-1. It also binds CD274-specific antibodies. This CD274 dimer can be used as an antigen for in vitro assays and antibody screening, and as an immunogen for immunization to generate antibodies targeting more conformational epitopes.
Product Name: CD274, Protein Dimer
UniProt #: G7PSE7
Predicted Molecular Weight: 67 kDa
SDS PAGE Molecular Weight: The migration range of the dimer protein with glycosylation under non-reducing condition is ~120 kDa on SDS PAGE.
Protein Construct: CD274 dimer protein contains a CD274 extracellular domainfused with a proprietary dimer motif followed by a His tag at the C-terminus.

Background and Alternate Names

Cluster of differentiation 274 (CD274), is a Type I transmembrane protein in the immunoglobulin superfamily and a member of the B7 Family of ligands. CD274 is also known as programmed death-ligand 1 (PD-L1), B7 homolog 1 (B7H1, B7-H1), PDCD1L1, and PDCD1LG1. CD274 contains an extracellular domain with a distal immunoglobulin V-like (Ig-V-like) domain and proximal immunoglobulin C-like (Ig-C-like) domain, a transmembrane domain, and a cytoplasmic domain. CD274 is expressed on T cells, NK cells, macrophages, myeloid DCs, B cells, epithelial cells, and vascular endothelial cells. CD274 serves as an immunosuppressive ligand for PD-1 and the overexpression of CD274 on many tumor cells can prevent the immune system from attacking tumors. Inhibition of the interaction between PD-1 and CD274 can enhance antitumor activity, which has led to a new class of drugs called PD-1 inhibitors to activate the immune system and treat certain types of cancer. CD274 is highly expressed in a variety of malignancies, particularly lung cancer. CD274 exists as a monomer but can form dimer. While structurally and functionally similar to human CD274 homodimer, Rhesus macaque CD274 homodimer is a species-specific tool essential for preclinical studies, basic research, and translational research.

Alternate Names: cluster of differentiation 274, CD274, B7-H, B7 homolog 1, B7H1, PDCD1L1, PDCD1LG1, PDL1, CD274 molecule, Programmed cell death ligand 1, hPD-L1