MW: Molecular Weight marker reduced condition NR: IFNγR1 dimer under non-reduced condition

Immobilized Rhesus macaque IFNγR1 protein dimer

His Tag (CSP-25295-01) at 2 μg/mL (100 μL/well) can bind anti-human IFNγR1 polyclonal antibody with half maximal effective concentration (EC50) range of 0.1-0.5 μg/mL (QC tested).

Bioactive, Rhesus macaque IFNγR1 Protein Dimer, His Tag

Cytokine Receptor

Product Code: CSP-25295-01

Expression Host: HEK293T

Verified Applications: SPR for interferon γ (IFNγ) ligand protein binding assays. ELISA for IFNγR1-specific antibody binding assays.

Suggested Applications: SPR & BLI for IFNγR1-specific antibody binding assays and BLI for IFNγ protein binding assays. Animal immunization, RUO.

Purity: Greater than 90% dimer form as determined by SDS-PAGE under non-reducing condition

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Formulation: 0.22μm filtered PBS, pH 7.4

Shipping: Frozen Dry Ice

Storage: -80’C

Rhesus macaque interferon gamma receptor 1 (IFNγR1) is a Type I integral membrane glycoprotein. The recombinant Rhesus macaque IFNγR1 dimer protein (CSP-25295-01) is a cis-homodimer (cis-dimer) and contains a IFNγR1 extracellular domain (UniProt# A0A5F8A7G4, amino acids Glu18-Gly245) fused with a proprietary dimer motif followed by a His tag at the C-terminus. This dimeric protein is expressed in HEK293T cells. The recombinant Rhesus macaque IFNγR1 dimer protein is bioactive and can bind to interferon γ (IFNγ) cytokine. It also binds IFNγR1-specific antibodies. This IFNγR1 dimer can be used as an antigen for in vitro assays and antibody screening, and as an immunogen for immunization to generate antibodies targeting more conformational epitopes.

Protein Name: IFNγR1

UniProt #: A0A5F8A7G4

Predicted Molecular Weight: 68 kDa

SDS PAGE Molecular Weight: The migration range of the heterodimer protein with glycosylation under non-reducing condition is ~120 kDa on SDS PAGE.

Protein Construct: IFNγR1 dimer protein contains a IFNγR1 extracellular domainfused with a proprietary dimer motif followed by a His tag at the C-terminus.

Background

Interferon gamma receptor 1 (IFNγR1), also known as cluster of differentiation 119 (CD119), IMD27A and IMD27B, is a subunit of interferon gamma receptor (IFNγR). IFNγR belongs to the type II cytokine receptor family. IFNγR1 is a Type I integral membrane glycoprotein containing extracellular, transmembrane and intracellular domains. IFNγR consists of two subunits: IFNγR1 (ligand-binding) and IFNγR2 (signal transduction). The extracellular domain has two immunoglobulin-like (Ig-like) C2-type domains. The interferon gamma (IFNγ) dimer interacts with two IFNγR1 molecules to activate the cascade signaling pathway. While structurally and functionally similar to human IFNγR1 homodimer, Rhesus macaque IFNγR1 homodimer is a species-specific tool essential for preclinical studies, basic research, and translational research.

Bioactive, Rhesus macaque IFNγR1 Protein Dimer, His Tag

Cytokine Receptor

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