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Human CD7 Protein Dimer, His Tag

Product Code: CSP-26322-01
Specificity: Human
Expression Host: HEK293T
Verified Applications: ELISA for CD7-specific antibody binding assays.
Suggested Applications: SPR & BLI for CD7-specific antibody binding assays and CAR-T detection. Animal immunization, RUO.
Purity: Greater than 90% dimer form as determined by SDS-PAGE under non-reducing condition
Amino Acid Range: A26 – P180

For Research Use Only (RUO)

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Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4 with 10% trehalose as protectant
Shipping: Frozen Ice Packs
Storage: -20’C
Reconstitution Information:

For best performance, we strongly recommend you reconsitute the lyophilized product with deionized water to a stock solution of 500 µg/mL.
Solubilize for 20 minutes at room temperature with occasional gentle mixing. Avoid shaking or vortexing.

For 25µg product size:
Reconstitute with 50 µL sterile deionized water.

For 100µg product size:
Reconstitute with 200 µL sterile deionized water.

Datasheet

MSDS

Human Cluster of Differentiation 7 (CD7) is a Type I transmembrane glycoprotein and a member of the immunoglobulin superfamily. The recombinant CD7 dimer protein (CSP-26322-01) is a cis-homodimer (cis-dimer) and contains a CD7 extracellular domain (UniProt# P09564, amino acids Ala26 – Pro180) fused with a proprietary cis-dimer motif followed by a His tag at the C-terminus. This dimeric protein is expressed in HEK293T cells. The recombinant human CD7 dimer protein is bioactive and can bind CD7-specific antibodies. This CD7 dimer can be used as an antigen for in vitro assays and antibody screening, and as an immunogen for immunization to generate antibodies targeting more conformational epitopes.
Product Name: CD7, Protein Dimer
Predicted Molecular Weight: 49 kDa
SDS PAGE Molecular Weight: The migration range of the protein dimer with glycosylation under non-reducing condition is 85-120 kDa on SDS-PAGE.
Protein Construct: CD7 dimer protein contains a CD7 extracellular domainfused with a proprietary cis-dimer motif followed by a His tag at the C-terminus.
Human Cluster of Differentiation 7 (CD7) is a Type I transmembrane glycoprotein and a member of the immunoglobulin superfamily. CD7 is also known as GP40, LEU-9, TP41, or Tp40. CD7 contains an extracellular region featuring a single IgV-like (immunoglobulin variable-like) domain. The extracellular region is followed by a proline-rich “stalk” that projects the domain away from the cell membrane. CD7 is expressed on T-cells, natural killer (NK) cells and their precursors. CD7 forms cis-homedimer on the cell surface creating a stable platform for ligand engagement. The Ig-like domain mediates cell-to-cell communication by engaging with natural ligands, primarily Galectin-1 and the K12. Upon binding its K12/SECTM1 ligand it acts in a costimulatory role in T-cell activation. CD7 serves as an excellent marker for the detection of T-cell lymphoproliferative disorders.

Alternate Names: GP40, LEU-9, TP41, Tp40