MW: Molecular Weight marker reduced condition
NR: DLK1 monomer under non-reduced condition
Immobilized human DLK1-His monomer protein (Cat. No. CSP-26440-01) at 2 μg/mL (100 μL/well) can bind anti-human DLK1monoclonal antibody with half maximal effective concentration (EC50) range of 6.5-25.8 ng/mL (QC tested).

Human DLK1 Protein Monomer, His Tag

Product Category: All Products

Product Code: CSP-26440-01
Specificity: Human
Expression Host: HEK293T
Verified Applications: ELISA for DLK1-specific antibody binding.
Suggested Applications: SPR & BLI for DLK1-specific antibody binding assays. Animal immunization, RUO.
Purity: Greater than 90% target protein as determined by SDS-PAGE under non-reducing condition
Amino Acid Range: A24-Q303

For Research Use Only (RUO)

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Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4 with 10% trehalose as a protectant
Shipping: Frozen Ice Packs
Storage: On receipt, store at -20 C or lower for long term storage. This product is stable at: -20 to -70 C for 12 months from date of receipt in lyophilized state. -70 C for 3 months after reconstitution under sterile conditions. Avoid repeated freeze-thaw cycles
Reconstitution Information:

For best performance, we strongly recommend you reconstitute the lyophilized product with deionized water to a stock solution of 500 µg/mL.
Solubilize for 20 minutes at room temperature with occasional gentle mixing. Avoid shaking or vortexing.

For 25µg product size:
Reconstitute with 50 µL sterile deionized water.

For 100µg product size:
Reconstitute with 200 µL sterile deionized water.

Datasheet

MSDS

DLK1 (Delta-like non-canonical Notch ligand 1), also known as Pref-1 or FA1, is a Type 1 transmembrane glycoprotein. The recombinant DLK1 monomer protein (CSP-26440-01) contains the DLK1 extracellular domain (UniProt# P80370, amino acids Ala24-Gln303) fused with a His tag at the C-terminus. This protein is expressed in HEK293T cells. The recombinant human DLK1 monomer protein is bioactive and binds DLK1-specific antibodies. This DLK1 monomer can be used as an antigen for in vitro assays and antibody screening, and as an immunogen for immunization to generate antibodies targeting more conformational epitopes.
Product Name: DLK1, Protein Monomer
UniProt #: P80370
Predicted Molecular Weight: 31 kDa
SDS PAGE Molecular Weight: The migration range of the dimer protein with glycosylation under non-reducing condition is between 40-50 kDa on SDS PAGE.
Protein Construct: DLK1 monomer protein contains the DLK1 extracellular domainfused with a His tag at the C-terminus.

Background and Alternate Names

DLK1 (Delta-like non-canonical Notch ligand 1), also known as Pref-1 or FA1, is a Type 1 transmembrane glycoprotein. It plays a critical role in controlling cell differentiation, tissue growth, and stem cell maintenance. DLK1 is highly expressed during embryonic development and functions as a regulator of multiple developmental processes, including adipogenesis, myogenesis, neurogenesis, and endocrine organ formation. The extracellular domain of DLK1 contains six EGF-like repeats and can be proteolytically cleaved to generate a soluble form that acts through paracrine and endocrine mechanisms. Although structurally related to canonical Notch ligands, DLK1 primarily functions as a non-canonical signaling molecule and has been implicated in regulating pathways involved in stem cell fate and tissue regeneration. Dysregulation of DLK1 expression has been associated with metabolic disorders, developmental abnormalities, and several cancers, highlighting its importance as both a biological marker and a potential therapeutic target.

Alternate Names: Delta-like non-canonical Notch ligand 1, Protein delta homolog 1, DLK-1, pG2, Pref-1, FA1