MW: Molecular Weight marker reduced condition
NR: EphA2 dimer under non-reducing condition
Immobilized human EphA2 dimer protein,His Tag (Cat. No. CSP-26324-01) at 2 μg/mL (100 μL/well) can bind anti-human EphA2 monoclonal antibody,with half maximal effective concentration (EC50) range of 2.4-9.6 ng/mL (QC tested).

Human EphA2 Protein Dimer, His Tag

Product Code: CSP-26324-01
Specificity: Human
Expression Host: HEK293T
Verified Applications: ELISA for EphA2-specific antibody binding assays.
Suggested Applications: SPR & BLI for EphA2-specific antibody binding assays and CAR-T detection. Animal immunization, RUO.
Purity: Greater than 90% dimer form as determined by SDS-PAGE under non-reducing condition
Amino Acid Range: A24 – V537

For Research Use Only (RUO)

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Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4 with 10% trehalose as protectant
Shipping: Frozen Ice Packs
Storage: -20’C
Reconstitution Information:

For best performance, we strongly recommend you reconsitute the lyophilized product with deionized water to a stock solution of 500 µg/mL.
Solubilize for 20 minutes at room temperature with occasional gentle mixing. Avoid shaking or vortexing.

For 25µg product size:
Reconstitute with 50 µL sterile deionized water.

For 100µg product size:
Reconstitute with 200 µL sterile deionized water.

Datasheet

MSDS

Human ephrin type-A receptor 2 (EphA2) is a Type I transmembrane protein and a member of the ephrin receptor subfamily of the protein-tyrosine kinase family. The recombinant EphA2 dimer protein (CSP-26324-01) is a cis-homodimer (cis-dimer) and contains an EphA2 extracellular domain (UniProt# P29317, amino acids Ala24 – Val537) fused with a proprietary cis-dimer motif followed by a His tag at the C-terminus. This dimeric protein is expressed in HEK293T cells. The recombinant human EphA2 dimer protein is bioactive and can bind to EphA2-specific antibodies. This EphA2 dimer can be used as an antigen for in vitro assays and antibody screening, and as an immunogen for immunization to generate antibodies targeting more conformational epitopes.
Product Name: EphA2, Protein Dimer
Predicted Molecular Weight: 129 kDa
SDS PAGE Molecular Weight: The migration range of the dimer protein with glycosylation under non-reducing conditions is 120-190 kDa on SDS PAGE.
Protein Construct: EphA2 dimer protein contains an EphA2 extracellular domainfused with a proprietary cis-dimer motif followed by a His tag at the C-terminus.
Human ephrin type-A receptor 2 (EphA2) is a Type I transmembrane protein and a member of the ephrin receptor subfamily, representing the largest subfamily of receptor tyrosine kinase (RTK) family. EphA2 is also known as EPH receptor A2. EphA2 contains an extracellular domain consisting of a Cys-rich domain and 2 fibronectin type III repeats. It is highly conserved and primarily acts as a gatekeeper for cell-to-cell communication, regulating tissue development, cell migration, and structural homeostasis. The dimerization of EphA2 serves as the primary structural gateway controlling its dual roles as a tumor suppressor and an oncogene. EphA2 binds ephrin-A ligands residing on adjacent cells, leading to contact-dependent signaling into neighboring cells. EphA2 is a receptor by which Kaposi’s sarcoma-associated herpesvirus (KSHV) enters host cells and is a prognostic marker in some cancers, making it an emerging target for therapeutics.

Alternate Names: EPH receptor A2, AW545284, Myk2, Sek-2, Sek2, ARCC2, CTPA, CTPP1, CTRCT6, ECK