MW: Molecular Weight marker reduced condition
NR: MICA dimer under non-reduced condition
Immobilized human MICA-His dimer protein (CSP-26327-01) at 2 μg/mL (100 μL/well) can bind anti-human MICA monoclonal antibody with half maximal effective concentration (EC50) range of 3.5-14.2 ng/mL (QC tested).

Human MICA Protein Dimer, His Tag

Product Code: CSP-26327-01
Specificity: Human
Expression Host: HEK293T
Verified Applications: ELISA for MICA-specific antibody binding assays.
Suggested Applications: SPR & BLI for MICA-specific antibody binding assays and CAR-T detection. Animal immunization, RUO.
Purity: Greater than 90% dimer form as determined by SDS-PAGE under non-reducing condition
Amino Acid Range: E24 – W307

For Research Use Only (RUO)

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Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4 with 10% trehalose as protectant
Shipping: Frozen Ice Packs
Storage: -20’C
Reconstitution Information:

For best performance, we strongly recommend you reconsitute the lyophilized product with deionized water to a stock solution of 500 µg/mL.
Solubilize for 20 minutes at room temperature with occasional gentle mixing. Avoid shaking or vortexing.

For 25µg product size:
Reconstitute with 50 µL sterile deionized water.

For 100µg product size:
Reconstitute with 200 µL sterile deionized water.

Datasheet

MSDS

Human MHC class I polypeptide–related sequence A (MICA) is a MHC class I family protein. It is a type I transmembrane protein. The recombinant MICA dimer protein (CSP-26327-01) is a cis-homodimer (cis-dimer) and contains a MICA extracellular domain (UniProt# Q29983, amino acids Glu24 – Trp307) fused with a proprietary cis-dimer motif followed by a His tag at the C-terminus. This dimeric protein is expressed in HEK293T cells. The recombinant human MICA dimer protein is bioactive and can bind to MICA-specific antibodies. This MICA dimer can be used as an antigen for in vitro assays and antibody screening, and as an immunogen for immunization to generate antibodies targeting more conformational epitopes.
Product Name: MICA, Protein Dimer
Predicted Molecular Weight: 82 kDa
SDS PAGE Molecular Weight: Migration range of the dimer under non-reducing condition is 120-190 kDa on SDS PAGE
Protein Construct: MICA dimer protein contains a MICA extracellular domainfused with a proprietary cis-dimer motif followed by a His tag at the C-terminus.
Human MHC class I polypeptide–related sequence A (MICA) is a MHC class I family protein. MICA is also known as PERB11.1 and MHC class I chain–related protein A. MICA functions as a stress-induced ligand for integral membrane protein receptor natural-killer group 2, member D (NKG2D). MICA is a type 1 transmembrane protein. The expression is highly regulated, and minimally expressed in most healthy tissues and strongly induced by cellular stress. MICA is constitutively expressed at low levels in certain epithelial tissues that are frequently exposed to environmental stress. MICA is frequently overexpressed in response to oncogenic stress, DNA damage, and genomic instability. Some tumors shed large amounts of MICA and high levels of MICA in the serum can be indicative of tumor growth with the amount of MICA present positively correlated to tumor size and poor prognosis. This behavior has made MICA an emerging target for cancer immunotherapy and cell therapy.

Alternate Names: MIC-A, PERB11.1, MHC class I chain–related protein A, MHC class I polypeptide–related protein A