MW: Molecular Weight marker reduced condition
NR: ROR2 dimer under non-reduced condition
Immobilized human ROR2 dimer protein,His Tag (Cat. No. CSP-25288-01) at 2 μg/mL (100 μL/well) can bind anti-human ROR2 monoclonal antibody,with half maximal effective concentration (EC50) range of 72.4-289.6 ng/mL (QC tested).

Human ROR2 Protein Dimer, His Tag

Product Code: CSP-25288-01
Expression Host: HEK293T
Verified Applications: ELISA for ROR2-specific antibody binding assays.
Suggested Applications: SPR & BLI for ROR2-specific antibody and Wnt-5a protein binding assays, and flow cytometry for ROR2-specific CAR (chimeric antigen receptor) expressing cell detection. Animal immunization, RUO.
Purity: Greater than 90% dimer form as determined by SDS-PAGE under non-reducing condition
Amino Acid Range: E34 – G403

For Research Use Only (RUO)

Add to Quote

Ready to buy now?
Email us at sales@conigen.com and we’ll help you place your order directly.

Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4 with 10% trehalose as a protectant
Shipping: Product is shipped at ambient temperature.
Storage: On receipt, store at -20 C or lower for long term storage. This product is stable at: -20 to -70 C for 12 months from date of receipt in lyophilized state. -70 C for 3 months after reconstitution under sterile conditions. Avoid repeated freeze-thaw cycles
Reconstitution Information:

For best performance, we strongly recommend you reconstitute the lyophilized product with deionized water to a stock solution of 500 µg/mL.
Solubilize for 20 minutes at room temperature with occasional gentle mixing. Avoid shaking or vortexing.

For 25µg product size:
Reconstitute with 50 µL sterile deionized water.

For 100µg product size:
Reconstitute with 200 µL sterile deionized water.

Datasheet

MSDS

Human tyrosine-protein kinase transmembrane receptor ROR2 (ROR2) is a Type I transmembrane protein and member of the receptor tyrosine kinase-like orphan receptor (ROR) family. The recombinant ROR2 dimer protein (CSP-25288-01) is a cis-homodimer (cis-dimer) and contains the ROR2 extracellular domain (UniProt# Q01974, amino acids Glu34 – Gly403) fused with a proprietary cis-dimer motif followed by a His tag at the C-terminus. This dimeric protein is expressed in HEK293T cells. The recombinant human ROR2 dimer protein is bioactive and can bind to Wnt-5a. It also binds ROR2-specific antibodies. This ROR2 dimer can be used as an antigen for in vitro assays and antibody screening, and as an immunogen for immunization to generate antibodies targeting more conformational epitopes.
Product Name: ROR2, Protein Dimer
Predicted Molecular Weight: 99 kDa
SDS PAGE Molecular Weight: The migration range of the dimer protein with glycosylation under non-reducing condition is 120-190 kDa on SDS PAGE.
Protein Construct: ROR2 dimer protein contains the ROR2 extracellular domainfused with a proprietary cis-dimer motif followed by a His tag at the C-terminus.
Human tyrosine-protein kinase transmembrane receptor ROR2 (ROR2) is a Type I transmembrane protein and member of the receptor tyrosine kinase-like orphan receptor (ROR) family. ROR2 is also known as neurotrophic tyrosine kinase, receptor-related 2. ROR2 contains an extracellular domain with an immunoglobulin (Ig)-like domain, a Frizzled-like cysteine-rich domain, and a Kringle domain. ROR2 is involved in the early formation of chondrocytes. ROR2 is thought to be a receptor for Wnt-5a and its binding inhibits of Wnt-3a-mediated signaling. ROR2 regulates cell migration, polarity, differentiation, and tissue development. While expression is limited in many normal adult tissues, ROR2 can be elevated in cancers, including melanoma, breast, ovarian, renal, lung, and pancreatic cancers, where it may promote invasion and metastasis. Upregulation of ROR2 in several cancers, such as breast and liver cancer, has made it an attractive emerging target in cancer therapies including monoclonal antibodies, antibody-drug conjugates, bispecific antibodies, and CAR-T therapies.

Alternate Names: BDB, BDB1, NTRKR2, receptor tyrosine kinase like orphan receptor 2