MW: Molecular Weight marker reduced conditionNR: Siglec 2 dimer under non-reduced condition
Immobilized human Siglec 2/CD22 protein dimer, His Tag (CSP-25199-01) at 2 μg/mL (100 μL/well) can bind anti-human Siglec 2 monoclonal antibody with half maximal effective concentration (EC50) range of 12-48 ng/mL (QC tested).

Human Siglec 2 / CD22 Protein Dimer, His Tag

Product Category: Siglec Receptors

Product Code: CSP-25199-01
Expression Host: HEK293T
Verified Applications: ELISA for Siglec 2/CD22-specific antibody binding assays.
Suggested Applications: SPR & BLI for Siglec 2/CD22-specific antibody binding assays. Animal immunization, RUO.
Purity: Greater than 90% dimer form as determined by SDS-PAGE under non-reducing condition
Amino Acid Range: D20-R687

For Research Use Only (RUO)

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Formulation: 0.22μm filtered PBS, pH 7.4
Shipping: Frozen Dry Ice
Storage: -80’C

Datasheet

MSDS

Human Sialic acid-binding Ig-like lectin 2 (Siglec 2/CD22) is a Type I transmembrane protein and a member of the Siglec family and immunoglobulin superfamily. The recombinant Siglec 2/CD22 dimer protein (CSP-25199-01) is a cis-homodimer (cis-dimer) and contains a Siglec 2 extracellular domain (UniProt# P20273, amino acids Asp20-Arg687) fused with a proprietary cis-dimer motif followed by a His tag at the C-terminus. This dimeric protein is expressed in HEK293T cells. The recombinant human Siglec 2 dimer protein can potently bind Siglec 2/CD22-specific antibodies. This Siglec 2/CD22 dimer can be used as an antigen for in vitro assays including antibody screening CAR-T cell detection, and as an immunogen for immunization to generate antibodies targeting more conformational epitopes.
Product Name: Siglec 2, Protein Dimer
UniProt #: P20273
Predicted Molecular Weight: 166 kDa
SDS PAGE Molecular Weight: The migration range of the homodimer protein with glycosylation under non-reducing conditions is greater than 190 kDa on SDS PAGE.
Protein Construct: Siglec 2/CD22 dimer protein contains a Siglec 2 extracellular domainfused with a proprietary cis-dimer motif followed by a His tag at the C-terminus.

Background and Alternate Names

Human Sialic acid-binding Ig-like lectin 2 (Siglec 2) is a member of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. Siglec 2 is also known as Cluster of Differentiation (CD22). It’s part of the immunoglobulin superfamily, and a multifunctional immune regulator in the innate immune system. Siglec 2/CD22 is a Type I transmembrane protein and can form homodimers on the cell surface. It contains an extracellular domain with an N-terminal V-type immunoglobulin domain (Ig domain) and six C2-type Ig domains followed by a transmembrane domain and cytoplasmic signaling domain consisting of the immunoreceptor tyrosine-based inhibitory motif (ITIM). Siglec 2/CD22 is highly expressed on B cells and expressed to a lesser extent on dendritic cells and mast cells. It binds sialylated glycans on cell surfaces or secreted proteins, modulating cell-cell interactions and immune responses. Due to the expression of Siglec 2/CD22 being mainly restricted to B cells, it is an excellent target for immunotherapy of B cell malignancies.

Alternate Names: cluster of differentiation 22, CD22, SIGLEC-2, SIGLEC2