MW: Molecular Weight marker reduced condition
NR: TIGIT dimer under non-reducing condition
Immobilize TIGIT-Fc dimer protein (Cat. No. CSP-24028-04) at 2 μg/mL (100 μL/well) can bind anti-human TIGIT monoclonal antibody with half maximal effective concentration (EC50) range of 3.5-14 ng/mL (QC tested).
Immobilized human CD155 dimer protein, His Tag (Cat. No. CSP-24029-01) at 2 μg/mL (100 μL/well) can bind human TIGIT dimer protein (Cat. No. CSP-24028-04), with half maximal effective concentration (EC50) range of 13.1-52.4 ng/mL (QC tested).

Bioactive human TIGIT Protein Dimer, Fc Tag

Product Category: Immune Checkpoint

Product Code: CSP-24028-04
Expression Host: HEK293T
Verified Applications: ELISA for TIGIT-specific antibody, Nectin-4 ligand protein and CD155 receptor protein binding assays.
Suggested Applications: SPR & BLI for TIGIT-specific antibody, Nectin-4 ligand protein and CD155 receptor protein binding assays. Animal immunization, RUO.
Purity: Greater than 90% dimer form as determined by SDS-PAGE under non-reducing condition
Amino Acid Range: M22-P141

For Research Use Only (RUO)

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Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4 with 10% trehalose as a protectant
Shipping: Product is shipped at ambient temperature.
Storage: On receipt, store at -20 C or lower for long term storage. This product is stable at: -20 to -70 C for 12 months from date of receipt in lyophilized state. -70 C for 3 months after reconstitution under sterile conditions. Avoid repeated freeze-thaw cycles

Datasheet

MSDS

Human TIGIT (T-cell immunoreceptor with Ig and ITIM domains) is also known as VSIG9, VSTM3. TIGIT protein dimer (CSP-24028-04) and contains a TIGIT extracellular domain (amino acids Met22-Phe141, UniProt# Q495A1) with a homodimer Fc tag at the C-terminus. This protein is expressed in HEK293 cells. The TIGIT-Fc dimer protein is bioactive and can bind to Nectin-4. It also binds to TIGIT-specific antibodies. This TIGIT dimer can be used as an antigen for in vitro assays and antibody screening, targeting more conformations epitopes.
Product Name: TIGIT, Protein Dimer
UniProt #: Q495A1
Predicted Molecular Weight: 104 kDa
SDS PAGE Molecular Weight: The migration range of the dimer under non-reducing condition is 85-150 kDa on SDS PAGE.
Protein Construct: TIGIT protein dimer contains a TIGIT extracellular domainwith a homodimer Fc tag at the C-terminus.

Background and Alternate Names

Human TIGIT (T-cell immunoreceptor with Ig and ITIM domains) is also known as VSIG9 (V-set and immunoglobulin domain-containing protein 9), VSTM3 (V-set and transmembrane domain-containing protein 3). TIGIT is a type I membrane protein containing an immunoglobulin variable (Ig-V) domain, a transmembrane domain and cytoplasmic domain. TIGIT is an immune receptor present on peripheral memory and regulatory CD4+ T cells and natural killer (NK) cells. TIGIT binds to CD155 (the poliovirus receptor, PVR) with high affinity and binds to CD112 (PVRL2) with lower affinity. Nectin-4 is also a ligand for TIGIT.

Alternate Names: VSIG9, VSTM3