MW: Molecular Weight marker reduced condition NR: TIGIT dimer under non-reducing condition
Immobilized TIGIT-His dimer protein (Cat. No. CSP-24028-01) at 2 μg/mL (100 μL/well) can bind anti-human TIGIT monoclonal antibody with half maximal effective concentration (EC50) range of 4.1-16.6 ng/mL (QC tested).

Human TIGIT Protein Dimer, His Tag

Immune checkpoint

Product Code: CSP-24028-01
Expression Host: HEK293T
Verified Applications: ELISA for TIGIT-specific antibody and Nectin-4 ligand protein binding assays.
Suggested Applications: ELISA for TIGIT-specific antibody, Nectin-4 ligand protein, and CD155 receptor protein binding assays. SPR & BLI for TIGIT-specific antibody, Nectin-4 ligand protein, and CD155 receptor protein binding assays. Animal immunization, RUO.
Purity: Greater than 90% dimer form as determined by SDS-PAGE under non-reducing condition
Amino Acid Range: M22-P141

For Research Use Only (RUO)

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Formulation: 0.22μm filtered PBS, pH 7.4
Shipping: Frozen Dry Ice
Storage: -80’C

Datasheet

MSDS

Human TIGIT (T-cell immunoreceptor with Ig and ITIM domains) is also known as VSIG9 and VSTM3. The recombinant TIGIT dimer protein (CSP-24028-01) is a cis-homodimer (cis-dimer) and contains a TIGIT extracellular domain (UniProt# Q495A1, amino acids Met22-Phe141) fused with a proprietary dimer motif followed by a His-tag at the C-terminus. This protein is expressed in HEK293T cells. The TIGIT-His dimer protein is bioactive and can bind to Nectin-4 and CD155. It also binds to TIGIT-specific antibodies. This TIGIT dimer can be used as an antigen for in vitro assays and antibody screening, targeting more conformational epitopes.
Product Name: TIGIT
UniProt #: Q495A1
Predicted Molecular Weight: 42 kDa
SDS PAGE Molecular Weight: The migration range of the dimer under non-reducing conditions is 40-70 kDa on SDS PAGE.
Protein Construct: TIGIT protein dimer contains a TIGIT extracellular domainwith a His tag at the C-terminus.

Background and Alternate Names

Human TIGIT (T-cell immunoreceptor with Ig and ITIM domains) is also known as VSIG9 (V-set and immunoglobulin domain-containing protein 9) and VSTM3 (V-set and transmembrane domain-containing protein 3). TIGIT is a type I membrane protein containing an immunoglobulin variable (Ig-V) domain, a transmembrane domain and cytoplasmic domain. TIGIT is an immune receptor present on peripheral memory and regulatory CD4+ T cells and natural killer (NK) cells. TIGIT binds to CD155 (the poliovirus receptor, PVR) with high affinity and binds to CD112 (PVRL2) with lower affinity. Nectin-4 is also a ligand for TIGIT.

Alternate Names: VSIG9, VSTM3