MW: Molecular Weight marker reduced condition
NR: IL-7Rα/TSLP R heterodimer under non-reduced condition
R: IL-7Rα/TSLP R heterodimer under reduced condition
Mouse IL-7Rα/TSLP R Heterodimer, His and Strep Tag
Cytokine Receptor
Product Code: CSP-25242-A1B6
Expression Host: HEK293T
Verified Applications: ELISA for IL-7Rα and TSLP R-specific antibody binding assays.
Suggested Applications: SPR & BLI for IL-7Rα and TSLP R-specific antibody binding assays. Animal immunization, RUO.
Purity: Greater than 90% dimer form as determined by SDS-PAGE under non-reducing condition
Ready to buy now? Email us at [email protected] and we’ll help you place your order directly.
Interleukin-7 receptor subunit alpha (IL-7Rα) and thymic stromal lymphopoietin receptor (TSLP R) compose a protein heterodimeric receptor complex. The recombinant IL-7Rα/TSLP R protein (CSP-25242-A1B6) is a heterodimer and contains an IL-7Rα extracellular domain (UniProt# P16872, amino acids Glu21-Asp239) and a TSLP R extracellular domain (UniProt# Q8CII9, amino acids Ala20-Pro232) fused with a proprietary cis-dimer motif followed by a His tag at the IL-7Rα C-terminus and a Strep tag at the TSLP R C-terminus. This heterodimeric protein is expressed in HEK293T cells. The recombinant mouse IL-7Rα/TSLP R heterodimer binds IL-7Rα and TSLP R-specific antibodies. This IL-7Rα/TSLP R heterodimer can be used as an antigen for in vitro assays and antibody screening, and as an immunogen for immunization to generate antibodies targeting more conformational epitopes.
Protein Name: IL-7Rα / TSLP R
UniProt #: AA: P16872
Predicted Molecular Weight: 63 kDa
SDS PAGE Molecular Weight: The migration range of the protein heterodimer with glycosylation under non-reducing condition is ~120 kDa and under reducing condition between 40 kDa for TSLP R chain and 50 kDa for IL-7Rα chain on SDS PAGE.
Protein Construct: IL-7Rα/TSLP R heterodimer protein contains an IL-7Rα extracellular domainand a TSLP R extracellular domainfused with a proprietary dimer motif followed by a His tag at the IL-7Rα C-terminus and a Strep tag at the TSLP R C-terminus.
Background
Interleukin-7 receptor subunit alpha (IL-7Rα) and thymic stromal lymphopoietin receptor (TSLP R) compose a protein heterodimeric receptor complex that binds the pro-inflammatory cytokine TSLP. IL-7Rα, also known as Cluster of Differentiation 127 (CD127), is a transmembrane receptor belonging to the cytokine receptor homology class 1 (CRH1) family with an extracellular domain consisting of two fibronectin type III (FNIII) domains (D1 and D2). TSLP R, also known as Cytokine receptor-like factor 2 (CRLF2), contains an extracellular domain consisting of a single CRH module composed of two tandem fibronectin type III (FnIII) domains (D1 and D2). The IL-7Rα/TSLP R heterodimer is central to the development of widespread allergic diseases, including asthma and atopic dermatitis, making it an attractive therapeutic target. While structurally and functionally similar to human IL-7Rα/TSLP R heterodimer, mouse IL-7Rα/TSLP R heterodimer is a species-specific tool essential for preclinical studies, basic research, and translational research in cancer immunotherapy.
MW: Molecular Weight marker reduced condition
NR: IL-7Rα/TSLP R heterodimer under non-reduced condition
R: IL-7Rα/TSLP R heterodimer under reduced condition