MW: Molecular Weight marker reduced condition
NR: CD20 dimer under non-reduced condition
Immobilized human CD20 Extracellular Loop 2 (ECL2) Protein,Dimer,His Tag (CSP-26403.S4-01) at 2 μg/mL (100 μL/well) can bind anti-human CD20 Rituximab biosimilar monoclonal antibody with half maximal effective concentration (EC50) range of 6.3-25 ng/mL (QC tested).
Flow cytometry analysis of anti-CD20 rituximab CAR expression on HEK293T cells transiently transfected with CD20-specific rituximab scFv CAR construct. Transfected cells were stained with PE-conjugated anti-G4S linker antibody and Human CD20-ECL2 Protein Dimer His Tag (CSP-26403.S4-01) followed by APC-conjugated anti-His tag antibody detection. Thereafter,flow cytometry analyses were performed to detect surface expression of the anti-CD20 CAR. The purple curve represents CD20 CAR expressing cells detected by Human CD20-ECL2 Protein Dimer His Tag (CSP-26403.S4-01) while the black curve represents no CD20 protein staining. Mock transfected cells have no binding to either anti-G4S linker antibody or Human CD20-ECL2 Protein Dimer His Tag. The histogram shows that anti-CD20 CAR are detected by Human CD20-ECL2 Protein Dimer His Tag (CSP-26403.S4-01) on anti-G4S linker positive cells

Soluble Human CD20 Extracellular Loop 2 (ECL2) Protein, Dimer, His Tag

Product Code: CSP-26403.S4-01
Specificity: Human
Expression Host: HEK293T
Verified Applications: ELISA for CD20-specific antibody and flow cytometry for detection of CD20-specific CAR (chimeric antigen receptor) expressing cells.
Suggested Applications: SPR & BLI for CD20-specific antibody binding assays. Animal immunization, RUO.
Purity: Greater than 90% dimer form as determined by SDS-PAGE under non-reducing condition
Amino Acid Range: H157-Y184

For Research Use Only (RUO)

Add to Quote

Ready to buy now?
Email us at sales@conigen.com and we’ll help you place your order directly.

Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4 with 10% trehalose as a protectant
Shipping: Product is shipped at ambient temperature.
Storage: On receipt, store at -20 C or lower for long term storage. This product is stable at: -20 to -70 C for 12 months from date of receipt in lyophilized state. -70 C for 3 months after reconstitution under sterile conditions. Avoid repeated freeze-thaw cycles
Reconstitution Information:

For best performance, we strongly recommend you reconstitute the lyophilized product with deionized water to a stock solution of 500 µg/mL.
Solubilize for 20 minutes at room temperature with occasional gentle mixing. Avoid shaking or vortexing.

For 25µg product size:
Reconstitute with 50 µL sterile deionized water.

For 100µg product size:
Reconstitute with 200 µL sterile deionized water.

Datasheet

MSDS

Human B-lymphocyte antigen CD20 (CD20), also known as MS4A1, is a transmembrane protein and a member of the membrane-spanning 4A gene family. The recombinant CD20 extracellular loop 2 (ECL2) dimer protein (CSP-26403.S4-01) is a cis-homodimer (cis-dimer) and contains CD20 extracellular loop 2 (UniProt# P11836, amino acids His157-Tyr184) fused with a proprietary scaffold cis-dimer motif followed by a His tag at the C-terminus. This dimeric protein is expressed in HEK293T cells. The recombinant human CD20-ECL2 dimer protein can bind anti-CD20 rituximab and CAR construct expressed on HEK293T cells transiently transfected with CD20-specific rituximab scFv CAR construct. This CD20 dimer can be used as an antigen for in vitro assays including antigen-specific CAR-T detection and antibody screening, and as an immunogen for immunization to generate antibodies targeting more conformational epitopes.
Product Name: CD20, Protein Dimer
Predicted Molecular Weight: 23 kDa
SDS PAGE Molecular Weight: The migration range of the dimer under non-reducing conditions is 25-40 kDa on SDS PAGE.
Protein Construct: CD20 dimer protein contains a CD20 extracellular loop 2fused with a proprietary scaffold cis-dimer motif followed by a His tag at the C-terminus.
Human B-lymphocyte antigen CD20 (CD20), also known as MS4A1, is a transmembrane protein and a member of the membrane-spanning 4A gene family. CD20 is a 4-transmembrane B-cell surface protein. CD20 contains two extracellular loops (ECL1 and ECL2): a shorter ECL1 and a larger ECL2. The ECL2 is the dominant antibody targeting region (1, 2). It presents on the cell surface as homo-dimeric and homo-tetrameric oligomers where it physically interacts with major histocompatibility complex class II (MHCII), CD40, and B-cell receptor (BCR). CD20 is expressed on pre-B phase B cells but not terminally differentiated plasma cells. CD20 expression is concentrated in B-cell–derived cancers, especially mature B-cell non-Hodgkin lymphomas. It is generally a high-density, stable surface antigen in many B-cell lymphomas, although expression varies by tumor type and can decrease after CD20-directed treatment.CD20 is one of the most clinically validated B-cell therapeutic targets and has become an attractive target for autoimmune diseases. For CD20, antibody-based therapeutics have evolved from conventional monoclonal antibodies into engineered antibodies, bispecific T-cell engagers, antibody drug conjugates, and CAR T cell therapies. 1. Carlson AK, et al. Drugs Targeting CD20 in Multiple Sclerosis: Pharmacology, Efficacy, Safety, and Tolerability. Drugs. 2024; 84(3):285–304 2. Klein C, et al. Epitope interactions of monoclonal antibodies targeting CD20 and their relationship to functional properties. mAbs. 2013; 5:22–33.

Alternate Names: MS4A1, B1, Bp35, CD20, CVID5, LEU-16, MS4A2, S7, membrane spanning 4-domains A1, FMC7