Immobilized human PAR1 extracellular domain loop 1 at 2 μg/mL (100 μL/well) can bind Mouse Anti-Human PAR1 Monoclonal Antibody (Cat. No. CABh-24076) with half maximal effective concentration (EC50) range of 12.1-48.2 ng/mL (QC tested).
Detection of PAR1 in HEK293 human cell line transiently transfected with human PAR1 by Flow Cytometry. HEK293 cell line transfected with human PAR1 was stained with isotype control (black curve) or Mouse Anti-Human PAR1 Monoclonal Antibody (Cat. No. CABh-24076) (green curve) by APC-conjugated Anti-Mouse IgG Secondary Antibody.
Immobilized human PAR1 extracellular domain loop 1 can bind Mouse Anti-Human PAR1 Monoclonal Antibody (Cat. No. CABh-24076) with a KD of 4.8-19.1 nM as determined by SPR.

Anti-Human PAR1 Monoclonal Antibody

Product Category: Antibody

Product Code: CABh-24076
Isotype: IgG2b
Conjugate: Unconjugated
Specificity: Human proteinase-activated receptor 1 / PAR1
Host Species: Mouse
Immunogen: PAR1, the exact sequence of the immunogen is proprietary; UniProt # P25116
Purification: Affinity Enrichment
Verified Applications: ELISA, Flow cytometry, LSPR
Purity: >90% as determined by SDS-PAGE under non-reducing condition
Recommended Usage: ELISA: starting concentration 1 µg/mL Flow cytometry: 0.5 µg/1E6 cells LSPR: Recommended maximum concentration, 200 nM

For Research Use Only (RUO)

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Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4 with 10% trehalose as a protectant
Shipping: Frozen Ice Packs
Storage: On receipt, store at -20 C or lower for long term storage. This product is stable at: -20 to -70 C for 12 months from date of receipt in lyophilized state. -70 C for 3 months after reconstitution under sterile conditions. Avoid repeated freeze-thaw cycles

Datasheet

MSDS

Structure: 7-transmembrane protein, G protein-coupled receptor (GPCR)
Function: Mediate coagulation and inflammation
Ligand/Receptor: thrombin, activated protein C
Cell Type: platelets and endothelial cells
Molecular Family: G protein-coupled receptor
Gene ID: P25116
Product Name: PAR1, Monoclonal Antibody
UniProt #: P25116;P30558
Predicted Molecular Weight: N/A
Protein Construct: N/A

Background and Alternate Names

Human proteinase-activated receptor 1 (PAR1), also known as thrombin receptor, is a G protein-coupled receptor (GPCR) on the cell surface with seven transmembrane domains. PAR1 is activated by thrombin cleavage of its extracellular propeptide at the N-terminus. PAR1 is highly expressed on platelets and endothelial cells. PAR1 is involved in the regulation of thrombotic response and plays a key role in mediating coagulation and inflammation, which is crucial in the pathogenesis of inflammatory and fibrotic lung diseases.

Alternate Names: Protease-activated receptor 1, coagulation factor II receptor, thrombin receptor, F2R, TR, PAR-1, CHTR