GHR (Growth Hormone Receptor) Proteins
Growth hormone receptor (GHR) is a type I cytokine receptor family member and the transmembrane receptor for growth hormone. GHR contains an extracellular domain with two fibronectin type III β domains, a single-pass transmembrane region, and an intracellular cytoplasmic domain that mediates downstream signaling. GHR can form preformed and growth hormone–induced dimers, and this dimerization is critical for activation of JAK2 signaling. Conigen provides recombinant GHR proteins designed to support binding studies, assay development, and therapeutic discovery, including formats that enable investigation of biologically relevant receptor interactions.
Example QC data shown. See individual product pages and datasheets for target-specific QC results.
Available formats
Soluble GHR ectodomain dimer CSPâ„¢ (Conigen Soluble Protein)
- Human (His-Avi)
Available GHR Products
| Product | Tag | Species | Product Code | Details | hf:tax:product_tag | hf:tax:pa_species |
|---|---|---|---|---|---|---|
| GHR | His-Avi | Human | CSP-24088-03 | Show details | his-avi | human |
Applications
- Antibody discovery and characterization (ELISA screening and specificity testing for GHR-targeting antibodies)
- Growth hormone binding studies (ELISA and SPR workflows to evaluate growth hormone–GHR interactions, including competition/blocked-binding formats)
- Binding kinetics and affinity studies> (SPR and BLI assays for GHR interactions with antibodies and growth hormone ligand)
- Assay and reagent development (workflow optimization and QC for GHR binding assays and screening reagents)
- Animal immunization (RUO) (as an immunogen to support generation of GHR-targeting antibodies)
Quality Control and Specifications
Quality Control Testing
- Purity assessment (greater than 90% dimer formed as determined by SDS-PAGE under reducing and non-reducing conditions).
- Bioactivity testing (binding activities confirmed by ELISA and ligand interaction assays: human growth hormone binding and GHR-specific antibody binding; SPR/BLI kinetics reported).
- Lot-specific documentation provided in downloadable product datasheets.
Product Specifications
- Format: Single-pass transmembrane, extracellular domain cis-dimers
- Expression host: HEK293T (mammalian)
- Construct: soluble extracellular domain (ECD) dimer fused with a proprietary dimer motif to mimic the native conformation
- Formulation: 0.22 μm filtered PBS, pH 7.4
- Storage & shipping: shipped frozen on dry ice; store at −80°C
About Conigen Soluble Proteins
Engineered dimeric proteins with quaternary structures
- Enhances receptor/ligand binding
- Increase functional activity
- Improve binding avidity for better detection sensitivity
- Compact size dimer motif
- Fc-free to avoid FcR binding
- Glycan-free dimer motifs to avoid target epitope masking
FAQs
What is GHR used for in research?
Recombinant GHR is widely used in research and drug development as a tool to study growth hormone-receptor interactions and support the development of growth hormone-related drugs. Common applications include:
- Ligand-receptor binding studies (ELISA, SPR, BLI)
- Drug discovery and developmentÂ
- Assay and method development
Is GHR provided as a monomer or dimer?
Our GHR is provided as a dimer and engineered to replicate the native, cell-surface conformation of GHR more closely than the monomeric ectodomain.
In practice, our dimeric GHR provides:
- Higher binding potency
- Improved assay sensitivityÂ
- Wider assay windows
Can GHR proteins be used in binding assays?
Yes, our recombinant GHR has been validated for use in binding assays, including ELISA-based GHR-specific antibody binding assays and ELISA- and SPR-based ligand binding assays.
How should GHR protein be stored and reconstituted?
GHR is supplied in PBS and is shipped on dry ice. Upon receipt, it should be stored at – 80°C. For optimal stability we recommend aliquoting the protein into smaller volumes before freezing to avoid repeated freeze-thaw cycles.
For short term use, the protein may be stored at 4°C for a limited period. For long-term storage, keep the protein at -80°C for up to 12 months. Avoid vigorous mixing; gently invert or pipette to mix if needed.
What tag formats are available for GHR?
Conigen offers GHR proteins in the His-Avi-tagged format.
Additional tags such as Fc, Flag, HA, or Myc or formats such as biotinylation can be provided through custom expression to match your assay or conjugation needs.
Can I request a custom GHR format or tag?
Yes. GHR proteins can be customized through Conigen’s CSP ™ platform. For custom GHR formats (alternate tags, variants, or assay-specific constructs), email [email protected] or submit a request through our Custom Project form.
What is the lead time for ordering GHR proteins?
Lead time varies by format and scale. Catalog GHR can be ordered directly and will ship within 2-3 business days of sales order receipt.Â
Custom GHR and larger orders will follow a defined project workflow, but will generally be shipped 4-6 weeks following request. Contact us for a precise delivery estimate.
Not sure which GHR format is best for your assay?