TROP-2 (TACSTD2) Proteins
TROP-2 (TACSTD2) is a type I transmembrane glycoprotein expressed on epithelial cells and frequently overexpressed in solid tumors, where it is associated with cancer progression and poor prognosis. Conigen provides soluble recombinant TROP-2 proteins to support antibody binding studies, assay development, and antibody discovery in oncology and translational research.
Example QC data shown. See individual product pages and datasheets for target-specific QC results.
Available formats
Soluble TROP-2 ectodomain dimer CSPâ„¢ (Conigen Soluble Protein)
- Human
- Mouse
Available tags
- FLAG-His
Available TROP-2 Products
| Product | Tag | Species | Product Code | Details | hf:tax:product_tag | hf:tax:pa_species |
|---|---|---|---|---|---|---|
| TROP-2 | Flag-His | Human | CSP-24041-02 | Show details | flag-his | human |
| TROP-2 | Flag-His | Mouse | CSP-25191-02 | Show details | flag-his | mouse |
Applications
- Antibody discovery and characterization(screening, specificity testing, and epitope recognition for TROP-2-targeting antibodies)
- Binding and affinity studies(ELISA, SPR, and BLI workflows for TROP-2 antibody binding and ranking)
- Assay and reagent development (ELISA development, assay optimization, and QC workflows for TROP-2 reagents)
- Animal immunization (RUO) (as an immunogen to support generation of TROP-2-targeting antibodies)
Quality Control and Specifications
Quality Control Testing
- Purity assessment (SDS-PAGE; >90% dimer under non-reducing conditions)
- Bioactivity testing (TROP-2–specific antibody binding by ELISA with EC50 reported per datasheet)
- Lot-specific documentation provided in downloadable product datasheets
Product Specifications
- Format: extracellular domain cis-dimers of TROP-2
- Expression host: HEK293T (mammalian)
- Construct: soluble extracellular domain (ECD) dimer fused with a proprietary dimer motif to mimic the native conformation
- Formulation: 0.22 μm filtered PBS, pH 7.4
- Storage & shipping: shipped frozen on dry ice; store at −80°C
Â
About Conigen Soluble Proteins
Engineered dimeric proteins with quaternary structures
- Increase functional activity
- Improve binding avidity for better detection sensitivity
- Compact size dimer motif
- Fc-free to avoid FcR binding
- Glycan-free dimer motifs to avoid target epitope masking
Â
FAQs
What is TROP-2 used for in research?
Recombinant TROP-2 is widely used as a research tool to study cancer biology, cell signaling, and therapeutic targeting as it is overexpressed in many epithelial cancers. Common applications include:
- Antibody and drug development
- Cell signaling and functional studies
- Assay and method development
Is TROP-2 provided as a monomer or dimer?
Our TROP-2 is provided as a dimer and engineered to replicate the native, cell-surface conformation of TROP-2 more closely than the monomeric ectodomain.
In practice, our dimeric TROP-2 provides:
- Higher binding potency
- Improved assay sensitivityÂ
- Wider assay windows
Can TROP-2 proteins be used in binding assays?
Yes, our recombinant TROP-2 has been validated for use in binding assays, including ELISA-based TROP-2-specific antibody binding assays.
How should TROP-2 protein be stored and reconstituted?
TROP-2 is supplied in PBS and is shipped on dry ice. Upon receipt, it should be stored at – 80°C. For optimal stability we recommend aliquoting the protein into smaller volumes before freezing to avoid repeated freeze-thaw cycles.
For short term use, the protein may be stored at 4°C for a limited period. For long-term storage, keep the protein at -80°C for up to 12 months. Avoid vigorous mixing; gently invert or pipette to mix if needed.
What tag formats are available for TROP-2?
TROP-2 is available from Conigen in both the human and mouse forms, each supplied with a His-Flag tag.
Â
Additional tags such as Fc, Avi, HA, or Myc or formats such as biotinylation can be provided through custom expression to match your assay or conjugation needs.
Can I request a custom TROP-2 format or tag?
Yes. TROP-2 proteins can be customized through Conigen’s CSP ™ platform. For custom TROP-2 formats (alternate tags, variants, or assay-specific constructs), email [email protected] or submit a request through our Custom Project form.
What is the lead time for ordering TROP-2 proteins?
Lead time varies by format and scale. Catalog TROP-2 can be ordered directly and will ship within 2-3 business days of sales order receipt.Â
Custom TROP-2 and larger orders will follow a defined project workflow, but will generally be shipped 4-6 weeks following request. Contact us for a precise delivery estimate.
Need help selecting the right TROP-2 format?