CXCR5 Proteins
CXCR5 is a GPCR chemokine receptor that binds CXCL13 chemokine and supports immune cell migration and follicular immune responses. Conigen provides full-length, multi-pass recombinant CXCR5 embedded in a native-like lipid bilayer on virus-like particles (VLPs) to support receptor/ligand and antibody binding studies, assay development, and antibody discovery for difficult membrane targets.
Example QC data shown. See individual product pages and datasheets for target-specific QC results.
Available formats
Full-length CXCR5 on VLP CMPâ„¢ (Conigen Membrane Protein)
- Standard
- Biotinylated
Available CXCR5 Products
| Product | Tag | Species | Product Code | Details | hf:tax:product_tag | hf:tax:pa_species |
|---|---|---|---|---|---|---|
| Biotinylated CXCR5 | Human | BCMP-24014 | Show details | human | ||
| CXCR5 | Human | CMP-24014 | Show details | human |
Applications
- Antibody discovery and characterization (screening, specificity testing, and binding to native-like CXCR5 epitopes)
- Receptor/Ligand-binding studies (CXCL13–CXCR5 interaction workflows, including competition/blocked-binding formats)
- Assay and reagent development (ELISA development, antibody binding assays, and workflow optimization for difficult GPCR targets)
- Animal immunization (as an immunogen to support generation of CXCR5-targeting antibodies)
Quality Control and Specifications
Quality Control Testing
- Identity + consistency testing performed per lot
- Bioactivity testing (e.g., antibody binding EC50 for validated antibodies)
- Particle characterization by DLS (size distribution / intensity)
Product Specifications
Format: CMPâ„¢ full-length membrane protein displayed on VLP nanoparticles
Expression host: HEK293T (mammalian)
Construct: full-length multi-pass receptor embedded in lipid bilayer
Formulation: 0.45μm filtered PBS, pH 7.4
Storage & shipping: shipped frozen on dry ice; store at -80°C
About Conigen Membrane Proteins
Engineered to enhance target expression level and VLP release
- Incorporated in a natural lipid bilayer
- Preserve the multi-pass protein conformation
- Enable receptor/ligand binding
- Bind to target-specific antibodies
Â
Choosing the right CXCR5 configuration
Conigen CXCR5 is supplied as a full-length, multi-pass membrane protein embedded in a native-like lipid bilayer on VLPs, designed specifically for challenging GPCR workflows. While the CXCR5 construct is consistent across products, you can select the configuration best suited to your assay setup:
Biotinylated vs Standard
Choose a biotinylated format if you:
- perform flow cytometry
- use streptavidin-based surfaces or beads
Choose a standard (non-biotinylated) format if you:
- use the protein in solid or solution-based workflows
- are optimizing or comparing native binding behavior in free solution
Not sure which to choose? If your workflow involves ELISA screening or assay development, the biotinylated format is typically preferred for consistent capture and presentation.
FAQs
What is CXCR5 used for in research?
Because CXCR5 plays an important role in immune cell trafficking, germinal center biology, autoimmunity, and oncology, recombinant CXCR5 displayed on virus-like particles (VLPs) is valuable for immunology and translational research focused on B cells, T follicular helper cells, and and is well suited for applications including:
- Antibody discovery and characterization
- Ligand-binding studies
- Assay and reagent development
How is CXCR5 presented in this product?
The full-length CXCR5 protein is incorporated into lipid-based virus-like particles (VLPs), preserving native transmembrane structure and extracellular epitopes for improved biological relevance compared to soluble or truncated forms.
What are the size of the virus-like particles?
The VLPs typically measure between 150-200 nm in diameter as confirmed by dynamic light scattering and maintain protein presentation in a native-like conformation.
Why use VLP-displayed CXCR5 instead of soluble protein?
VLP display preserves the native membrane context of CXCR5,which is critical for maintaining correct conformation of extracellular loops and ligand-binding sites. This format can improve assay relevance, antibody recognition, and functional binding compared to soluble or truncated constructs.
How should CXCR5 be stored and handled?
CXCR5 is supplied in PBS and is shipped on dry ice. Upon receipt, it should be stored at – 80°C. For optimal stability we recommend aliquoting the protein into smaller volumes before freezing to avoid repeated freeze-thaw cycles.
For short term use, the protein may be stored at 4°C for a limited period. For long-term storage, keep the protein at -80°C for up to 12 months. Avoid vigorous mixing; gently invert or pipette to mix if needed.
What formats is CXCR5 available in?
CXCR5 is available in both non-biotinylated and biotinylated VLP formats. The biotinylated format enables convenient capture and detection in streptavidin-based assays, including ELISA, bead-based assays, and other affinity-based platforms.
What is the lead time for ordering CXCR5 proteins?
Lead time varies by format and scale. Catalog CXCR5 can be ordered directly and will ship within 2-3 business days of sales order receipt.Â
Custom CXCR5 and larger orders will follow a defined project workflow, but will generally be shipped 4-6 weeks following request. Contact us for a precise delivery estimate.
Is a negative control available for CXCR5 experiments?
Yes, a matched negative control VLP (particle without CXCR5) is available and recommended for use as an experimental control. This control contains the same scaffold and formulation as the CXCR5-displayed product but does not express CXCR5, enabling accurate assessment of CXCR5-specific binding and background signal.
Need help selecting the right CXCR5 format?