Bioactive, Recombinant Human GHR Protein Dimer, His-Avi Tag
| Product Code | CSP-24088-03 |
| Expression Host | HEK293T |
| Verified Applications | ELISA for GHR-specific antibodies and ELISA and SPR for growth hormone ligand protein binding assays. |
| Suggested Applications | SPR and BLI for GHR-specific antibodies and BLI for growth hormone protein binding assays. Animal immunization, RUO. |
| Purity | Greater than 90% dimer form as determined by SDS-PAGE under non-reducing condition |
| Amino Acid Range | F19-Y264 |
For Research Use Only (RUO)
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Bioactivity – Antibody Binding
Immobilized human GHR protein dimer, His Tag (CSP-24088-03) at 2 μg/mL (100 μL/well) can bind anti-human GHR monoclonal antibody with half maximal effective concentration (EC50) range of 17.4-69.8 ng/mL (QC tested).
Bioactivity – Ligand Binding
Immobilized human Growth Hormone at 2 μg/mL (100 μL/well) can bind human GHR protein dimer, His Tag (CSP-24088-03) with half maximal effective concentration (EC50) range of 5.7-22.8 ng/mL (QC tested).
SDS-PAGE
NR: GHR dimer under non-reduced condition
Specifications
Formulation: 0.22μm filtered PBS, pH 7.4
Shipping: Frozen Dry Ice
Storage: -80°C
Human growth hormone receptor (GHR) is the transmembrane protein receptor for growth hormone and a member of the Type I cytokine receptor family of receptors. The recombinant GHR protein dimer (CSP-24088-03) is a cis-homodimer (cis-dimer) and contains a GHR extracellular domain (UniProt# P10912, amino acids Phe19-Tyr264) fused with a proprietary cis-dimer motif followed by a tandem His-Avi tag at the C-terminus. This dimeric protein is expressed in HEK293T cells. The recombinant human GHR dimer protein is bioactive and can bind to growth hormone. It also binds GHR-specific antibodies. This GHR dimer can be used as an antigen for in vitro assays and antibody screening, and as an immunogen for immunization to generate antibodies targeting more conformational epitopes.
Protein Name: GHR
UniProt #: P10912
Predicted Molecular Weight: 76 kDa
SDS PAGE Molecular Weight: The migration range of the heterodimer protein with glycosylation under non-reducing condition is between 120 and 190 on SDS PAGE.
Protein Construct: GHR protein dimer contains a GHR extracellular domain (UniProt# P10912) fused with a proprietary dimer motif followed by a tandem His-Avi tag at the C-terminus.
Background
Human growth hormone receptor (GHR) is the transmembrane protein receptor for growth hormone and a member of the Type I cytokine receptor family of receptors. GHR is also known as GH binding protein (GHBP) and GHIP. GHR contains an extracellular domain with two fibronectin type III β domains followed by a single-pass transmembrane domain and a cytoplasmic intracellular domain. GHR can form dimers as preformed dimer and growth hormone induced dimer. GHR dimerization is crucial for growth hormone signaling to activate an intracellular enzyme Janus kinase 2 (JAK2). GHR is widely distributed in the body, and the preformed GHR homodimer is expressed across a wide range of cellular types in different tissues. Mutations in the GHR gene have been associated with growth hormone insensitivity syndrome (GHIS) and growth hormone plays a significant role in cancer development. A recombinant protein mimicking the GHR dimer conformation can be crucial for therapeutic innovation.
Alternate Names: GH binding protein, GHBP, GHIP