Bioactive, Recombinant Human IL-10Rα/IL-10Rβ Protein heterodimer, His and Strep Tag
| Product Code | CSP-25251-A1B6 |
| Expression Host | HEK293T |
| Verified Applications | ELISA for IL-10Rα and IL-10Rβ-specific antibodies and interleukin-10 (IL-10) ligand protein binding assays. |
| Suggested Applications | SPR & BLI for IL-10Rα and IL-10Rβ-specific antibodies and IL-10 protein binding assays. Animal immunization, RUO. |
| Purity | Greater than 90% dimer form as determined by SDS-PAGE under non-reducing condition |
| Amino Acid Range | H22-N235 ; M20-S220 |
For Research Use Only (RUO)
Price: $125.00
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Price: $3,500.00
Bioactivity – Antibody Binding
Immobilized human IL-10Rα/IL-10Rβ protein heterodimer, His and Strep-tag (CSP-25251-AB) at 2 μg/mL (100 μL/well) can bind anti-human IL-10Rβ monoclonal antibody with half maximal effective concentration (EC50) range of 8.4-33.7 ng/mL (QC tested).
Bioactivity – Antibody Binding
Immobilized human IL-10 at 2 μg/mL (100 μL/well) can bind human IL-10Rα/IL-10Rβ protein heterodimer, His and Strep-tag (CSP-25251-AB) with half maximal effective concentration (EC50) range of 27.5-109.9 ng/mL (QC tested).
SDS-PAGE
NR: IL-10Rα/IL-10Rβ heterodimer under non-reduced condition
R: IL-10Rα/IL-10Rβ heterodimer under reduced condition
Specifications
Formulation: 0.22μm filtered PBS, pH 7.4
Shipping: Frozen Dry Ice
Storage: -80°C
Human interleukin-10 receptor (IL-10R) is a heterodimer belonging to the type II cytokine receptor family. The recombinant IL-10R protein (CSP-25251-AB) is a heterodimer and contains an IL-10Rα extracellular domain (UniProt# Q13651, amino acids His22-Asn235) and an IL-10Rβ extracellular domain (UniProt# Q08334, amino acids Met20-Ser220) fused with a proprietary dimer motif followed by a His tag at the IL-10Rα C-terminus and a Strep tag at the IL-10Rβ C-terminus. This heterodimeric protein is expressed in HEK293T cells. The recombinant human IL-10R heterodimer protein is bioactive and can bind to interleukin-10 (IL-10) cytokine. It also binds IL-10Rα and IL-10Rβ-specific antibodies. This IL-10R heterodimer can be used as an antigen for in vitro assays and antibody screening, and as an immunogen for immunization to generate antibodies targeting more conformational epitopes.
Protein Name: IL10Ra / IL10Rb
UniProt #: AA: Q13651 ; BA: Q08334
Predicted Molecular Weight: 63 kDa
SDS PAGE Molecular Weight: The migration range of the heterodimer protein with glycosylation under non-reducing condition is between 120-190 kDa and between 50-60 kDa under reducing condition on SDS PAGE.
Protein Construct: IL-10R protein heterodimer contains an IL-10Rα extracellular domain (UniProt# Q08334) and an IL-10Rβ extracellular domain (UniProt# Q13651) fused with a proprietary dimer motif followed by a His tag at the IL-10Rα C-terminus and a Strep tag at the IL-10Rβ C-terminus.
Background
Human interleukin-10 receptor (IL-10R) is a heterodimer belonging to the type II cytokine receptor family, mediating the anti-inflammatory effects of interleukin-10 (IL-10), a key cytokine involved in limiting immune responses and preventing tissue damage. The IL-10R protein heterodimer is composed of interleukin-10 receptor alpha (IL-10Rα) and interleukin-10 receptor beta (IL-10Rβ). IL-10Rα is a Type I integral membrane glycoprotein containing an extracellular domain with two immunoglobulin-like (Ig-like) C2-type domains. IL-10Rβ has two β-sandwich domains (D1 and D2), a β-hairpin structure, and a “thumb-like” structure. IL-10Rα is a subunit unique to IL-10R while IL-10Rβ is shared with other type II cytokine receptors such as IL-22R, IL-28R, and INFλR. The IL-10Rα subunit interacts with IL-10 and IL-10Rβ acts as the signaling subunit. IL-10R is involved with human gastro-intestinal immune response and defects in IL-10R are correlated with the development of irritable bowel disease. Therefore, a recombinant protein mimicking the IL-10R heterodimer conformation can be very useful for research and therapeutic discovery for cancer, autoimmune diseases, and inflammatory conditions.
Alternate Names: IL-10 R1, cluster of differentiation 210, CD210, CD210α, and CDW210A, IL10RB, cluster of differentiation w210b, CDW210B, CRF2-4, CRFB4, D21S58, D21S66, IL-10R2, interleukin 10 receptor subunit beta