MW: Molecular Weight marker reduced condition NR: IL-4Rα dimer under non-reduced condition

Immobilized human IL-4Rα protein dimer

His-tag (CSP-25219-01) at 2 μg/mL (100 μL/well) can bind anti-human IL-4Rα monoclonal antibody with half maximal effective concentration (EC50) range of 6-24 ng/mL (QC tested).

Bioactive, Human IL4Ra Protein Dimer, His Tag

Cytokine Receptor

Product Code: CSP-25219-01

Expression Host: HEK293T

Verified Applications: ELISA for IL-4Rα-specific antibody and interleukin 4 (IL-4) ligand protein binding assays.

Suggested Applications: SPR & BLI for IL-4Rα-specific antibody and IL-4 protein binding assays. Animal immunization, RUO.

Purity: Greater than 90% dimer form as determined by SDS-PAGE under non-reducing condition

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Formulation: 0.22μm filtered PBS, pH 7.4

Shipping: Frozen Dry Ice

Storage: -80’C

Human interleukin 4 receptor alpha (IL-4Rα) is a Type I transmembrane and a Type I cytokine receptor. The recombinant IL-4Rα protein dimer (CSP-25219-01) is a cis-homodimer (cis-dimer) and contains a IL-4Rα extracellular domain (UniProt# P24394, amino acids Met26-His232) fused with a proprietary cis-dimer motif followed by a His tag at the C-terminus. This dimeric protein is expressed in HEK293T cells. The recombinant human IL-4Rα protein dimer is bioactive and can bind to interleukin 4 (IL-4). It also binds IL-4Rα-specific antibodies. This IL-4Rα protein dimer can be used as an antigen for in vitro assays and antibody screening, and as an immunogen for immunization to generate antibodies targeting more conformational epitopes.

Protein Name: IL-4Rα

UniProt #: AA: P24394

Predicted Molecular Weight: 64 kDa

SDS PAGE Molecular Weight: The migration range of the heterodimer protein with glycosylation under non-reducing condition is between 120 and 190 kDa on SDS PAGE.

Protein Construct: IL-4Rα dimer protein contains a IL-4Rα extracellular domainfused with a proprietary cis-dimer motif followed by a His tag at the C-terminus.

Background

Human interleukin 4 receptor alpha (IL-4Rα) is a Type I transmembrane and a Type I cytokine receptor. IL-4Rα is also known as cluster of differentiation 124 (CD124). IL-4Rα is a key component in interleukin 4 (IL-4) and IL-13 cytokine signaling involved in immune regulation, particularly in Th2 immune responses, allergy, and asthma. IL-4Rα contains an extracellular domain with an overall L shape organized in two covalently linked domains: an h-type immunoglobulin fold (D1) and a standard fibronectin type III (FN III)–like topology (D2). IL-4Rα can bind IL-4 and IL-13 and allergic inflammation is largely driven by IL-4 and IL-13 signaling through IL-4Rα, making IL-4Rα a promising therapeutic target for allergic diseases.

Bioactive, Human IL4Ra Protein Dimer, His Tag

Cytokine Receptor

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