Human Siglec 9 Protein Dimer, His Tag
| Product Code | CSP-25206-01 |
| Expression Host | HEK293T |
| Verified Applications | ELISA for Siglec 9-specific antibody binding assays. |
| Suggested Applications | SPR & BLI for Siglec 9-specific antibody binding assays. Animal immunization, RUO. |
| Purity | Greater than 90% dimer form as determined by SDS-PAGE under non-reducing condition |
| Amino Acid Range | Q18-G348 |
For Research Use Only (RUO)
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Bioactivity – Antibody Binding
Immobilized human Siglec 9 protein dimer, His Tag (CSP-25206-01) at 2 μg/mL (100 μL/well) can bind anti-human Siglec 9 monoclonal antibody with half maximal effective concentration (EC50) range of 6.3-25.3 ng/mL (QC tested).
SDS-PAGE
NR: Siglec 9 dimer under non-reduced condition
Specifications
Formulation: 0.22μm filtered PBS, pH 7.4
Shipping: Frozen Dry Ice
Storage: -80°C
Human Sialic acid-binding Ig-like lectin 9 (Siglec 9) is a Type I transmembrane protein and a member of the Siglec family and immunoglobulin superfamily. The recombinant Siglec 9 protein dimer (CSP-25206-01) is a cis-homodimer (cis-dimer) and contains a Siglec 9 extracellular domain (UniProt# Q9Y336, amino acids Gln18-Gly348) fused with a proprietary cis-dimer motif followed by a His tag at the C-terminus. This dimeric protein is expressed in HEK293T cells. The recombinant human Siglec 9 protein dimer can potently bind Siglec 9-specific antibodies. This Siglec 9 protein dimer can be used as an antigen for in vitro assays and antibody screening, and as an immunogen for immunization to generate antibodies targeting more conformational epitopes.
Protein Name: Siglec 9
UniProt #: Q9Y336
Predicted Molecular Weight: 88 kDa
SDS PAGE Molecular Weight: The migration range of the dimer protein with glycosylation under non-reducing condition is ~190 kDa on SDS PAGE.
Protein Construct: Siglec 9 dimer protein contains a Siglec 9 extracellular domain (UniProt# Q9Y336) fused with a proprietary cis-dimer motif followed by a His tag at the C-terminus.
Background
Human Sialic acid-binding Ig-like lectin 9 (Siglec 9) is a member of the Siglec family, part of the immunoglobulin superfamily, and a multifunctional immune regulator. It plays an important role in the immune system, especially in regulating inflammation and immune cell activation. Siglec 9 is also known as Cluster of Differentiation 329 (CD329), CDw329, FOAP-9, and OBBP-LIKE. Siglec 9 is a Type I transmembrane protein. It contains an extracellular domain with an N-terminal V-type immunoglobulin domain (Ig domain) and two C2-type Ig domains followed by a transmembrane domain and cytoplasmic signaling domain consisting of the immunoreceptor tyrosine-based inhibitory motif (ITIM). Siglec 9 is expressed on monocytes, neutrophils, and dendritic cells. It binds sialylated glycans on cell surfaces or secreted proteins, modulating cell-cell interactions and immune responses. Siglec 9 can form homodimers on the cell surface that plays a role in ligand binding, signal transduction, and receptor clustering. Some tumors express high levels of sialic acids to engage Siglec 9 and suppress immune attacks — potential immune evasion mechanism. The interaction of sialic acids with Siglecs is associated with suppression of anti-tumor responses has made Siglecs an emerging target for cancer therapeutics.
Alternate Names: Cluster of Differentiation 329, CD329, CDw329, FOAP-9, OBBP-LIKE, sialic acid binding Ig like lectin 9