Bioactive, Recombinant Human TrkC Protein Dimer, His Tag
| Product Code | CSP-25169-01 |
| Expression Host | HEK293T |
| Verified Applications | ELISA for TrkC-specific antibody and neurotrophin-3 (NT-3) ligand protein binding assays |
| Suggested Applications | SPR & BLI for TrkC-specific antibody and NT-3 protein binding assays. Animal immunization, RUO. |
| Purity | Greater than 90% dimer form as determined by SDS-PAGE under non-reducing condition |
| Amino Acid Range | C32-T429 |
For Research Use Only (RUO)
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Bioactivity – Antibody Binding
Immobilized human TrkC protein dimer, His-tag (CSP-25169-01) at 2 μg/mL (100 μL/well) can bind anti-human TrkC monoclonal antibody with half maximal effective concentration (EC50) range of 13-52.1 ng/mL (QC tested).
Bioactivity – Ligand Binding
Immobilized human NT-3 protein at 2 μg/mL (100 μL/well) can bind human TrkC protein dimer, His-tag (CSP-25169-01) with half maximal effective concentration (EC50) range of 14.4-57.4 ng/mL (QC tested).
SDS-PAGE
NR: TrkC dimer under non-reduced condition
Specifications
Formulation: 0.22μm filtered PBS, pH 7.4
Shipping: Frozen Dry Ice
Storage: -80°C
Human tropomyosin receptor kinase C (TrkC) is a transmembrane protein that is part of the family of receptor tyrosine kinases. The recombinant TrkC protein dimer (CSP-25169-01) is a cis-homodimer (cis-dimer) and contains the TrkC extracellular domain (UniProt# Q16288, amino acids Cys32-Thr429) fused with a proprietary cis-dimer motif followed by a His tag at the C-terminus. This dimeric protein is expressed in HEK293T cells. The recombinant human TrkC protein dimer is bioactive and can bind to neurotrophin-3 (NT-3). It also binds TrkC -specific antibodies. This TrkC protein dimer can be used as an antigen for in vitro assays and antibody screening, and as an immunogen for immunization to generate antibodies targeting more conformational epitopes.
Protein Name: TrkC
UniProt #: Q16288
Predicted Molecular Weight: 106 kDa
SDS PAGE Molecular Weight: The migration range of the dimer protein with glycosylation under non-reducing condition is ~190 kDa on SDS PAGE.
Protein Construct: TrkC protein dimer contains the TrkC extracellular domain (UniProt# Q16288) fused with a proprietary cis-dimer motif followed by a His tag at the C-terminus.
Background
Human tropomyosin receptor kinase C (TrkC) is part of the family of receptor tyrosine kinases and primarily the receptor for neurotrophin-3 (NT-3). TrkC is also known as neurotrophic receptor tyrosine kinase 3 (NTRK3), NT-3 growth factor receptor, neurotrophic tyrosine kinase receptor type 3, gp145, and GP145-TrkC. TrkC is a Type I transmembrane protein. It contains an extracellular domain with two cysteine-rich clusters (C1 and C2), three leucine-rich 24-residue repeats (LRR1–3), and two immunoglobulin-like domains (Ig1 and Ig2) followed by a transmembrane domain and a cytoplasmic domain. TrkC is a high affinity receptor for NT-3, and ligand binding induces receptor dimerization which is required for trans-autophosphorylation that triggers downstream signaling cascades. TrkC plays a role in many cancers, therefore, a recombinant protein mimicking the dimer conformation can be crucial for cancer therapeutic discovery.
Alternate Names: neurotrophic receptor tyrosine kinase 3, NTRK3, NT-3 growth factor receptor, neurotrophic tyrosine kinase receptor type 3, TrkC tyrosine kinase, gp145, GP145-TrkC, TRKC