Mouse tropomyosin receptor kinase C (TrkC) is a transmembrane protein that is part of the family of receptor tyrosine kinases. The recombinant TrkC protein dimer (CSP-25176-03) is a cis-homodimer (cis-dimer) and contains the TrkC extracellular domain (UniProt# Q6VNS1, amino acids Cys32-Thr429) fused with a proprietary dimer motif followed by a tandem His-Avi tag at the C-terminus. This dimeric protein is expressed in HEK293T cells. The recombinant mouse TrkC protein dimer is can bind TrkC-specific antibodies. This TrkC protein dimer can be used as an antigen for in vitro assays and antibody screening, and as an immunogen for immunization to generate antibodies targeting more conformational epitopes.
Protein Name: TrkC
UniProt #: 1: Q6VNS1
Predicted Molecular Weight: 109 kDa
SDS PAGE Molecular Weight: The migration range of the heterodimer protein with glycosylation under non-reducing condition is >190 kDa on SDS PAGE.
Protein Construct: TrkC protein dimer contains the TrkC extracellular domainfused with a proprietary cis-dimer motif followed by a tandem His-Avi tag at the C-terminus.
Background
Tropomyosin receptor kinase C (TrkC) is part of the family of receptor tyrosine kinases and primarily the receptor for neurotrophin-3 (NT-3). TrkC is also known as neurotrophic receptor tyrosine kinase 3 (NTRK3), NT-3 growth factor receptor, neurotrophic tyrosine kinase receptor type 3, gp145, and GP145-TrkC. TrkC is a Type I transmembrane protein. It contains an extracellular domain with two cysteine-rich clusters (C1 and C2), three leucine-rich 24-residue repeats (LRR1–3), and two immunoglobulin-like domains (Ig1 and Ig2) followed by a transmembrane domain and a cytoplasmic domain. TrkC is a high affinity receptor for NT-3, and ligand binding induces receptor dimerization which is required for trans-autophosphorylation that triggers downstream signaling cascades. TrkC plays a role in many cancers, therefore, a recombinant protein mimicking the dimer conformation can be crucial for cancer therapeutic discovery. While structurally and functionally similar to human TrkC homodimer, mouse TrkC homodimer is a species-specific tool essential for preclinical studies, basic research, and translational research.
MW: Molecular Weight marker reduced condition
NR: TrkC dimer under non-reduced condition
Immobilized mouse TrkC protein dimer