Rhesus macaque IL6ST Protein Dimer, His Tag
| Product Code | CSP-25296-01 |
| Expression Host | HEK293T |
| Verified Applications | ELISA for IL6ST-specific antibody binding assays. |
| Suggested Applications | SPR & BLI for IL6ST-specific antibody binding assays. Animal immunization, RUO. |
| Purity | Greater than 90% dimer form as determined by SDS-PAGE under non-reducing condition |
| Amino Acid Range | E47-E643 |
For Research Use Only (RUO)
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Bioactivity – Antibody Binding
Immobilized Rhesus macaque IL6ST protein dimer, His Tag (Cat. No. CSP-25296-01) at 2 μg/mL (100 μL/well) can bind anti-non-human primate IL6ST monoclonal antibody with half maximal effective concentration (EC50) range of 5.7-22.9 ng/mL (QC tested).
SDS-PAGE
MW: Molecular Weight marker reduced condition
NR: IL6ST dimer under non-reduced condition
Specifications
Formulation: 0.22μm filtered PBS, pH 7.4
Shipping: Frozen Dry Ice
Storage: -80°C
Interleukin 6 cytokine family signal transducer (IL6ST) is a transmembrane protein and a member of the class of tall cytokine receptors. The recombinant IL6ST dimer protein (CSP-25296-01) is a cis-homodimer (cis-dimer) and contains an IL6ST extracellular domain (UniProt# F7FXB6, amino acids Glu47-Glu643) fused with a proprietary cis-dimer motif followed by a His tag at the C-terminus. This dimeric protein is expressed in HEK293T cells. The recombinant Rhesus macaque IL6ST protein dimer is bioactive and can bind to interleukin-6 (IL-6). It also binds IL6ST-specific antibodies. This IL6ST dimer can be used as an antigen for in vitro assays and antibody screening, and as an immunogen for immunization to generate antibodies targeting more conformational epitopes.
Protein Name: IL6ST
UniProt #: F7FXB6
Predicted Molecular Weight: 152 kDa
SDS PAGE Molecular Weight: The migration range of the heterodimer protein with glycosylation under non-reducing conditions is >190 kDa on SDS PAGE.
Protein Construct: IL6ST dimer protein contains an IL6ST extracellular domain (UniProt# F7FXB6) fused with a proprietary dimer motif followed by a His tag at the C-terminus.
Background
Interleukin 6 cytokine family signal transducer (IL6ST) is a transmembrane protein and a member of the class of tall cytokine receptors. IL6ST is also known as interleukin-6 receptor subunit beta (IL-6Rb), Cluster of Differentiation 130 (CD130), CDW130, and glycoprotein 130 (gp130). IL6ST serves as a shared signal transducing subunit of the receptor complexes for Rhesus macaque cytokines, including interleukin-6 (IL-6), that mediate highly diverse biological processes. IL6ST can form homodimers and heterodimers with other cytokine receptors (i.e., IL-6 receptor alpha (IL-6Ra)) in response to cytokine binding. The homodimerization or heterodimerization of IL6ST is key to initiating intracellular signaling pathways. The extracellular domain of IL6ST includes an N-terminal immunoglobulin-like (Ig-like) domain (D1), a cytokine-binding homology region (CHR, D2D3), and three membrane-proximal fibronectin type III domains (FNIII, D4 to D6) followed by a transmembrane domain and cytoplasmic domain. It has been found that dysregulation of IL6ST expression and signaling mediates progression for multiple types of cancer and autoimmune diseases. Inhibition of IL6ST activity offers a potential and promising approach to cancer and autoimmune disease therapy. While structurally and functionally similar to human IL6ST (also known as gp130) homodimer, Rhesus macaque IL6ST homodimer is a species-specific tool essential for preclinical studies, basic research, and translational research.
Alternate Names: Cluster of Differentiation 130, CD130, CDW130, glycoprotein 130, GP130, Interleukin-6 receptor subunit beta, IL-6R-beta IL6Rb, IL-6RB, IL-6R beta, IL6β, interleukin 6 signal transducer